DLK1 Antibody Cat. No.: 7017

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: DLK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DLK1.

The immunogen is located within amino acids 260 - 310 of DLK1.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: DLK1 antibody can be used for detection of DLK1 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least four isoforms of DLK1 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1211 - HepG2 Cell Lysate
 2) Cat. No. 17-011 - Hep G2 Cell Slide
PREDICTED MOLECULAR WEIGHT: 42 kDa

psi-iconProperties
PURIFICATION:DLK1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DLK1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DLK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:DLK1
ALTERNATE NAMES:DLK1 Antibody: DLK, Protein delta homolog 1, pG2, DLK-1
ACCESSION NO.:NP_003827
PROTEIN GI NO.:74136023
GENE ID:8788
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:DLK1 Antibody: The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes and is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child and adolescent obesity.
REFERENCES:1) Smas CM and Sul HS. Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation. Cell 1993; 73:725-34.
2) Sul HS. Mini-review: Pref-1: role in adipogenesis and mesenchymal cell fate. Mol. Endocrinol. 2009; 23:1717-25.
3) Kawakami T, Chano T, Minami K, et al. Imprinted DLK1 is a putative tumor suppressor gene and inactivated by epimutation at the region upstream of GTL2 in human renal cell carcinoma. Hum. Mol. Genet. 2006; 15:821-30.
4) Wermter AK, Scherag A, Meyre D, et al. Preferential reciprocal transfer of paternal/maternal DLK1 alleles to obese children: first evidence of polar overdomainance in human. Eur. J. Hum. Genet. 2008; 16:1126-34.

ANTIBODIES FOR RESEARCH USE ONLY.

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