Disp2 Antibody Cat. No.: 4883

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Disp2 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human Disp2.

The immunogen is located within amino acids 1210 - 1260 of Disp2.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: Disp2 antibody can be used for the detection of Disp2 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate

psi-iconProperties
PURIFICATION:Disp2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Disp2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Disp2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:DISP2
ALTERNATE NAMES:Disp2 Antibody: DISPB, HsT16908, DISPB, KIAA1742, Protein dispatched homolog 2
ACCESSION NO.:A7MBM2
PROTEIN GI NO.:160380692
GENE ID:85455
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Disp2 Antibody: Disp2 is the second of three known homologs of the D. melanogaster protein Dispatched. It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored Hedgehog from producing cells. Hedgehog is a major player in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis and the Disp proteins have been implicated in these pathways. Recently, it has been shown that Disp2 is translationally regulated by the microRNA miR-214 in zebrafish. Expression of this miRNA decreased Disp2 promoter activity in vitro and its overexpression in zebrafish resulted in a phenotype identical to that observed by Disp2 mutants. At least two isoforms of Disp2 are known to exist. This Disp2 antibody is predicted to not cross-react with Disp1 or Disp3.
REFERENCES:1) Katoh Y and Katoh M. Identification and characterization of DISP3 gene in silico. Int. J. Oncol.2005; 26:551-6.
2) Katoh Y and Katoh M. Hedgehog signaling pathway and gastric cancer. Can. Biol. & Ther.2005; 4:1050-4.
3) Li N, Flynt AS, Kim HR, et al. Dispatched homolog 2 is targeted by miR-214 through a combination of three week microRNA recognition sites. Nuc. Acids Res.2008; 36:4277-85.

ANTIBODIES FOR RESEARCH USE ONLY.

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