Datasheet

DIS3L2 Antibody
CATALOG NUMBER: 8347

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:DIS3L2 antibody can be used for detection of DIS3L2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 97 kDa

Observed: 94 kDa
SPECIFICITY:DIS3L2 antibody is human, mouse and rat reactive. At least three isoforms of DIS3L2 are known to exist; this antibody will only detect the longest isoform. DIS3L2 is predicted to not cross-react with DIS3 or DIS3L.
IMMUNOGEN:DIS3L2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DIS3L2.

The immunogen is located within amino acids 720 - 770 of DIS3L2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DIS3L2 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DIS3L2 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DIS3L2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DIS3 like 3'-5' exoribonuclease 2, FAM6A, PRLMNS
ACCESSION NO.:NP_689596
PROTEIN GI NO.:134288890
OFFICIAL SYMBOL:DIS3L2
GENE ID:129563

Background

BACKGROUND:The exosome is involved in a multitude of cellular RNA processing and degradation events (1). DIS3, also known as exosome complex exonuclease RRP44, is a ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species (2). DIS3L2 is a paralog of DIS3, but unlike DIS3 is located in the cytoplasm (3). DIS3L2 defines a novel eukaryotic RNA degradation pathway and preferentially targets uridylated RNA (4). DIS3L2 mutations have also been associated with sporadic Wilms tumors (3).
REFERENCES: 1) Chen CY, Gherzi R, Ong SE, et al. AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. Cell 2001; 107:451-64.
2) Brouwer R, Allmang C, Raijmakers R, et al. Three novel components of the human exosome. J. Biol. Chem. 2001; 276:6177-84.
3) Astuti D, Morris MR, Cooper WN, et al. Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility. Nat. Genet. 2012; 44:277-84.
4) Malecki M, Viegas SC, Carneiro T, et al. The exonuclease Dis3L2 defines a novel eukaryotic RNA degradation pathway. EMBO J. 2013; 32:1842-54.

For Research Use Only