Datasheet

DIRAS3 Antibody
CATALOG NUMBER: 8139

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 25 kDa

Observed: 24 kDa
SPECIFICITY:DIRAS3 antibody is human specific.
IMMUNOGEN:DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3.

The immunogen is located within the last 50 amino acids of DIRAS3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DIRAS3 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DIRAS3 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DIRAS3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DIRAS family GTP-binding RAS-like 3, ARHI, NOEY2
ACCESSION NO.:NP_004666
PROTEIN GI NO.:4757772
OFFICIAL SYMBOL:DIRAS3
GENE ID:9077

Background

BACKGROUND:DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).
REFERENCES: 1) Yu Y, Xu F, Peng H, et al. NOEY2 (ARHI), an imprinted putative tumor suppressor gene in ovarian and breast carcinomas. Proc. Natl. Acad. Sci. USA 1999; 96:214-9.
2) Lu Z, Luo RZ, Lu Y, et al. The tumor suppressor gene ARHI regulates autophagy and tumor dormancy in human ovarian cancer cells. J. Clin. Invest. 2008; 118:3917-29.
3) Klingauf M, Beck M, Berge U, et al. The tumour suppressor DiRas3 interacts with C-RAF and downregulates MEK activity to restrict cell migration. Biol. Cell 2013; 105:91-107.

For Research Use Only