DIRAS3 Antibody Cat. No.: 8139

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3.

The immunogen is located within the last 50 amino acids of DIRAS3.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
SPECIFICITY: DIRAS3 antibody is human specific.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 25 kDa

Observed: 24 kDa

psi-iconProperties
PURIFICATION:DIRAS3 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DIRAS3 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DIRAS3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:DIRAS3
ALTERNATE NAMES:DIRAS family GTP-binding RAS-like 3, ARHI, NOEY2
ACCESSION NO.:NP_004666
PROTEIN GI NO.:4757772
GENE ID:9077
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).
REFERENCES:1) Yu Y, Xu F, Peng H, et al. NOEY2 (ARHI), an imprinted putative tumor suppressor gene in ovarian and breast carcinomas. Proc. Natl. Acad. Sci. USA 1999; 96:214-9.
2) Lu Z, Luo RZ, Lu Y, et al. The tumor suppressor gene ARHI regulates autophagy and tumor dormancy in human ovarian cancer cells. J. Clin. Invest. 2008; 118:3917-29.
3) Klingauf M, Beck M, Berge U, et al. The tumour suppressor DiRas3 interacts with C-RAF and downregulates MEK activity to restrict cell migration. Biol. Cell 2013; 105:91-107.

ANTIBODIES FOR RESEARCH USE ONLY.

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