Datasheet

DHX36 Antibody
CATALOG NUMBER: 7163

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:DHX36 antibody can be used for detection of DHX36 by Western blot at 0.5 - 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1404 - Mouse Liver Tissue Lysate
 2) Cat. No. 10-201 - Human Liver Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 111 kDa
SPECIFICITY:At least four isoforms of DDX36 are known to exist; this antibody will detect the three longest isoforms.
IMMUNOGEN:Rabbit polyclonal DHX36 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DHX36.

The immunogen is located within the last 50 amino acids of DHX36.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DHX36 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DHX36 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DHX36 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DHX36 Antibody: G4R1, RHAU, DDX36, MLEL1, KIAA1488, Probable ATP-dependent RNA helicase DHX36, DEAH box protein 36
ACCESSION NO.:NP_065916
PROTEIN GI NO.:167830433
OFFICIAL SYMBOL:DHX36
GENE ID:170506

Background

BACKGROUND:DHX36 Antibody: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure. DHX36 (DEAH box protein 36), also known as MLE-like protein 1 and RNA helicase associated with AU-rich element ARE (RHAU), belongs to RNA helicase of the DEAH family and may function in sex development and spermatogenesis. It is expressed in testis and is evolutionary conserved with true orthologs in almost all animal species. DHX36 plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. DHX36 is required for early embryogenesis.
REFERENCES: 1) Cordin O, Banroques J, Tanner NK, et al. The DEAD-box protein family of RNA helicases. Gene 2006; 367:17-37.
2) Linder P. Dead-box proteins: a family affair—active and passive players in RNP-remodeling. Nucleic Acids Res. 2006; 34:4168-80.
3) Lattmann S, Giri B, Vaughn JP, et al. Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU. Nucleic Acids Res. 2010; 38:6219-33.
4) Fu JJ, Li LY, Liu SF, et al. Expression research for human DDX36 and mouse Ddx36 gene in the adult testis. Yi Chuan Xue Bao 2003; 30: 201-208.

For Research Use Only