DHX36 Antibody Cat. No.: 7163

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: Rabbit polyclonal DHX36 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DHX36.

The immunogen is located within the last 50 amino acids of DHX36.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: DHX36 antibody can be used for detection of DHX36 by Western blot at 0.5 - 1 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: At least four isoforms of DDX36 are known to exist; this antibody will detect the three longest isoforms.
POSITIVE CONTROL:1) Cat. No. 1404 - Mouse Liver Tissue Lysate
 2) Cat. No. 10-201 - Human Liver Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 111 kDa

psi-iconProperties
PURIFICATION:DHX36 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DHX36 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DHX36 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:DHX36
ALTERNATE NAMES:DHX36 Antibody: G4R1, RHAU, DDX36, MLEL1, KIAA1488, Probable ATP-dependent RNA helicase DHX36, DEAH box protein 36
ACCESSION NO.:NP_065916
PROTEIN GI NO.:167830433
GENE ID:170506
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:DHX36 Antibody: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure. DHX36 (DEAH box protein 36), also known as MLE-like protein 1 and RNA helicase associated with AU-rich element ARE (RHAU), belongs to RNA helicase of the DEAH family and may function in sex development and spermatogenesis. It is expressed in testis and is evolutionary conserved with true orthologs in almost all animal species. DHX36 plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. DHX36 is required for early embryogenesis.
REFERENCES:1) Cordin O, Banroques J, Tanner NK, et al. The DEAD-box protein family of RNA helicases. Gene 2006; 367:17-37.
2) Linder P. Dead-box proteins: a family affair—active and passive players in RNP-remodeling. Nucleic Acids Res. 2006; 34:4168-80.
3) Lattmann S, Giri B, Vaughn JP, et al. Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU. Nucleic Acids Res. 2010; 38:6219-33.
4) Fu JJ, Li LY, Liu SF, et al. Expression research for human DDX36 and mouse Ddx36 gene in the adult testis. Yi Chuan Xue Bao 2003; 30: 201-208.

ANTIBODIES FOR RESEARCH USE ONLY.

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