Datasheet

DEPTOR Antibody
CATALOG NUMBER: 7787

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1404 - Mouse Liver Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 45 kDa

Observed: 42 kDa
SPECIFICITY:DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms.
IMMUNOGEN:DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR.

The immunogen is located within amino acids 220 - 270 of DEPTOR.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DEPTOR antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DEPTOR Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DEPTOR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DEPTOR Antibody: DEP.6, DEPDC6, DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6
ACCESSION NO.:NP_073620
PROTEIN GI NO.:189571664
OFFICIAL SYMBOL:DEPTOR
GENE ID:64798

Background

BACKGROUND:DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).
REFERENCES: 1) Peterson TR, Laplante M, Thoreen CC, et al. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for survival. Cell 2009; 137:873-6.
2) Duan S, Skaar JR, Kuchay S, et al. mTOR generates an auto-amplification loop by triggering the bTrCP- and CK1a-dependent degradation of DEPTOR. Mol. Cell 2011; 44:317-24.

For Research Use Only