| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Chicken: (100%), Bovine: (100%) |
| IMMUNOGEN: | DENR antibody was raised against an 18 amino acid peptide near the center of human DENR. The immunogen is located within amino acids 80 - 130 of DENR. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | DENR antibody can be used for detection of DENR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. |
| SPECIFICITY: | DENR antibody is human, mouse and rat reactive. |
| POSITIVE CONTROL: | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 22 kDa Observed: 22 kDa |
Specifications| PURIFICATION: | DENR antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | DENR Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | DENR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| OFFICIAL SYMBOL: | DENR |
| ALTERNATE NAMES: | DENR Antibody: DRP, DRP1, SMAP-3, H14, Density-regulated protein, Protein DRP1, DRP |
| ACCESSION NO.: | NP_003668 |
| PROTEIN GI NO.: | 27501446 |
| GENE ID: | 8562 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | DENR (density-regulated protein), also designated DRP or smooth muscle cell associated protein 3 (SMAP-3), is a 198 amino acid protein that may be involved in the translation of target mRNAs by scanning and recognition of the initiation codon (1). It plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1 (2,3). DENR contains a SUI1 domain and is found in a variety of tissues with highest levels present in skeletal and cardiac muscle. It is up-regulated in ovarian and breast cancer cells by ERBB2 overexpression (4). |
| REFERENCES: | 1) Deyo JE, Chiao PJ, Tainsky MA. DRP, a novel protein expressed at high cell density but not during growth arrest. DNA Cell Biol. 1998; 17:437-47. | 2) Skabkin MA, Skabkina OV, Dhote V, et al. Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling. Genes Dev. 2010; 24:1787-801. | 3) Mazan-Mamczarz K and Gartenhaus RB. Post-transcriptional control of the MCT1-associated protein DENR/DRP by RNA-binding protein AUF1. Cancer Genomics Proteomics 2007; 4:233-9. | 4) Oh JJ, Grosshans DR, Wong SG et al. Identification of differentially expressed genes associated with HER-2/neu overexpression in human breast cancer cells. Nucleic Acids Res. 1999; 27:4008-17. |
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