Datasheet

DELE Antibody
CATALOG NUMBER: 6919

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (71%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:DELE antibody can be used for detection of DELE by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
IMMUNOGEN:DELE antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human DELE.

The immunogen is located within the first 50 amino acids of DELE.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DELE Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DELE Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DELE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DELE Antibody: DELE, DELE, Death ligand signal enhancer
ACCESSION NO.:NP_055588
PROTEIN GI NO.:217330656
OFFICIAL SYMBOL:KIAA0141
GENE ID:9812

Background

BACKGROUND:DELE Antibody: DELE is a recently identified DAP3-binding protein that is thought to be important in the induction of death receptor-mediated apoptosis. Transfected cells that stably express DELE were found to be susceptible to apoptosis induction by TNF-α and TRAIL, whereas reducing DELE expression by siRNA rescued these cells from apoptosis induction. Furthermore, the reduction of DELE expression also inhibited the activation of caspase-3, caspase-8 and caspase-9 following stimulation by TNF-α, anti-Fas, or TRAIL, indicating the importance of DELE in apoptosis mediated by death receptors.
REFERENCES: 1) Harada T, Iwai A, Miyazaki T. Identification of DELE, a novel DAP-binding protein which is crucial for death receptor-mediated apoptosis induction. Apoptosis 2010; 15:1247-55.

For Research Use Only