DEDAF Antibody Cat. No.: 2227

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: DEDAF antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DEDAF.

The immunogen is located within the last 50 amino acids of DEDAF.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: DEDAF can be used for detection of DEDAF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1203 - A549 Cell Lysate
 2) Cat. No. 1211 - HepG2 Cell Lysate
 3) Cat. No. 1404 - Mouse Liver Tissue Lysate
 4) Cat. No. 17-003 - A549 Cell Slide
PREDICTED MOLECULAR WEIGHT: 32 kDa

psi-iconProperties
PURIFICATION:DEDAF Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DEDAF Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DEDAF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:RYBP
ALTERNATE NAMES:DEDAF Antibody: AAP1, DEDAF, YEAF1, RING1 and YY1-binding protein, Apoptin-associating protein 1, APAP-1
ACCESSION NO.:AF179286
PROTEIN GI NO.:5802963
GENE ID:23429
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:DEDAF Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
REFERENCES:1) Zheng L, Schickling O, Peter ME, et al. The death effector domain-associated factor (DEDAF) plays distinct regulatory roles in the nucleus and cytoplasm. J. Biol. Chem. 2001; 276:31945-52.
2) Garcia E, Marcos-Gutierrez C, del Mar Lorente M, et al. RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. EMBO J. 1999;18:3404-18.

ANTIBODIES FOR RESEARCH USE ONLY.

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