Datasheet

DDX60 Antibody
CATALOG NUMBER: 6945

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:DDX60 antibody can be used for detection of DDX60 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
 2) Cat. No. 17-208 - A-20 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 125, 185, 188 kDa

Observed: 190 kDa
SPECIFICITY:At least three isoforms of DDX60 are known to exist; this antibody will detect all three isoforms.
IMMUNOGEN:DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60.

The immunogen is located within amino acids 1140 - 1190 of DDX60.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DDX60 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DDX60 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DDX60 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DDX60 Antibody: Probable ATP-dependent RNA helicase DDX60, DEAD box protein 60
ACCESSION NO.:NP_060101
PROTEIN GI NO.:222831595
OFFICIAL SYMBOL:DDX60
GENE ID:55601

Background

BACKGROUND:DDX60 Antibody: DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.
REFERENCES: 1) Miyashita M, Oshiumi H, Matsumoto M, et al. DDX60, a DEXD/H box helicase, is a novel antiviral factor promoting RIG-I-like receptor-mediated signaling. Mol. Cell. Biol. 2011; 31:3802-19.

For Research Use Only