DDX5 Antibody Cat. No.: 45-470

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Expected Species Reactivity based on sequence homology: Rat, Dog
IMMUNOGEN: The immunogen for this antibody is: C-PMIGYPMPTGYSQ
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 70kDa band observed in nuclear lysates of cell lines NIH3T3, A431, HeLa and Jurkat (calculated MW of 69.1kDa according to NP_004387.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunofluorescence: A customer has reported successful staining at a dilution of 1:100 on HT-1080 cells with a Rhodamine conjugated secondary antibody. This data is from a previous batch, not on sale.
SPECIFICITY: #To_Delete#.
POSITIVE CONTROL:1) Cat. No. 1202 - A431 Cell Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:DDX5
ALTERNATE NAMES:DDX5, P68, HLR1, G17P1, HUMP68, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kDa), RNA-dependent ATPase, DEAD/H box-5 (RNA helicase, 68kD), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD), DEAD (Asp-Glu-Ala-Asp) box polypeptide 5, DKFZp434E109, DKFZp686J01190, ATP-dependent RNA helicase DDX5, DEAD box-5
ACCESSION NO.:NP_004387.1
PROTEIN GI NO.:4758138
GENE ID:1655
psi-iconBackground and References
REFERENCES:1) Iggo R, Gough A, Xu W, Lane DP, Spurr NK. Chromosome mapping of the human gene encoding the 68- kDa nuclear antigen (p68) by using the polymerase chain reaction. Proc Natl Acad Sci USA. 1989 Aug;86(16):6211-4.

ANTIBODIES FOR RESEARCH USE ONLY.

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