DDX41 Antibody Cat. No.: 7349

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: DDX41 antibody was raised against a 17 amino acid peptide near the amino terminus of human DDX41.

The immunogen is located within amino acids 230 - 280 of DDX41.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: DDX41 antibody can be used for detection of DDX41 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
SPECIFICITY: At least two isoforms of DDX41 are known to exist; this antibody will detect both isoforms.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 68 kDa

Observed: 70 kDa

psi-iconProperties
PURIFICATION:DDX41 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DDX41 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DDX41 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:DDX41
ALTERNATE NAMES:DDX41 Antibody: ABS, ABS, Probable ATP-dependent RNA helicase DDX41, DEAD box protein 41
ACCESSION NO.:NP_057306
PROTEIN GI NO.:21071032
GENE ID:51428
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:DDX41 Antibody: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DDX41, also known as Abstrakt, interacts with and regulates the expression of sorting nexin-2 (SNX2), a protein involved in protein sorting in the trans-Golgi network. Recent evidence suggests that DDX41 also plays a role in the innate immune response by sensing intracellular viral DNA, triggering TBK1 and IRF3 activation, leading to a type I interferon immune response.
REFERENCES:1) Cordin O, Banroques J, Tanner NK, et al. The DEAD-box protein family of RNA helicases. Gene 2006; 367:17-37.
2) Linder P. Dead-box proteins: a family affair—active and passive players in RNP-remodeling. Nucleic Acids Res. 2006; 34:4168-80.
3) Abdul-Ghani M, Hartman KL, and Ngsee JK. Abstrakt interacts with and regulates the expression of sorting nexin-2. J. Cell Physiol. 2005; 204:210-8.
4) Zhang Z, Yuan B, Bao M, et al. The helicase DDX41 senses intracellular DNA mediated by the adaptor STING in dendritic cells. Nat. Immunol. 2011; 12:959-65.

ANTIBODIES FOR RESEARCH USE ONLY.

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