Datasheet

DDDDK Antibody [1D1B12] (biotin)
CATALOG NUMBER: PM-7657-biotin

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Tag
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:Biotin-DDDDK antibody can be used for detection of DDDDK by Western blot at 0.5 - 1 μ/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) N/A
PREDICTED MOLECULAR WEIGHT:N/A
SPECIFICITY:Biotin-DDDDK-tag antibody will detect carboxy-terminal DDDDK-tagged recombinant proteins.
IMMUNOGEN:Biotin-DDDDK antibody was raised against a synthetic peptide containing DYKDDDDK.
HOST SPECIES:Mouse

Properties

PURIFICATION:Biotin-DDDDK antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Biotin-DDDDK antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Biotin-DDDDK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Monoclonal
ISOTYPE:IgG
CONJUGATE:Biotin

Additional Info

ALTERNATE NAMES:Biotin-DDDDK, DDDDK, FLAG, FLAGtag,flag-tag
ACCESSION NO.:N/A
PROTEIN GI NO.:N/A
OFFICIAL SYMBOL:ACTB
GENE ID:60

Background

BACKGROUND:Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. DDDDK-tag is one of the more popular epitope tags for expressed recombinant proteins.

For Research Use Only