Datasheet

DDC Antibody
CATALOG NUMBER: 7447

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (92%), Guinea pig: (77%), Pig: (77%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:DDC antibody can be used for detection of DDC by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1302 - Human Lung Tissue Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 53 kDa

Observed: 53 kDa
SPECIFICITY:DDC antibody is human, mouse and rat reactive.
IMMUNOGEN:DDC antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DDC.

The immunogen is located within amino acids 390 - 440 of DDC .
HOST SPECIES:Rabbit

Properties

PURIFICATION:DDC Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DDC Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DDC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DDC Antibody : AADC, AADC, Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase
ACCESSION NO.:NP_000781
PROTEIN GI NO.:4503281
OFFICIAL SYMBOL:DDC
GENE ID:1644

Background

BACKGROUND:DOPA decarboxylase (DDC) belongs to the group II decarboxylase family of proteins (1). It is an important protein in the catecholamine biosynthesis pathway. DDC catalyzes the second reaction in the biosynthesis of catecholamines, trace amines and serotonin (1,2). It can form a homodimer and is expressed in the central nervous system (2). DDC can be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease, neuroendocrine tumors and drug abuse (3,4). Defects in DDC gene may cause the autosomal recessive disorder AADC deficiency (4).
REFERENCES: 1) Berry MD, Juorio AV, Li XM, et al. Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem. Res. 1996; 21:1075-87.
2) Sumi-Ichinose C, Ichinose H, Takahashi E, et al. Molecular cloning of genomic DNA and chromosomal assignment of the gene for human aromatic L-amino acid decarboxylase, the enzyme for catecholamine and Serotonin biosynthesis. Biochemistry 1992; 31:2229-38.
3) Haycock JW, Becker L, Ang L, et al. Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J. Neurochem. 2003; 87:574-85.
4) Chang YT, Sharma R, Marsh JL, et al. Levodopa-responsive aromatic L-amino acid decarboxylase deficiency. Ann. Neurol. 2004; 55:435-8.

For Research Use Only