DDA Antibody Cat. No.: 6483

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (84%)
IMMUNOGEN: DDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DDA1.

The immunogen is located within the last 50 amino acids of DDA.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: DDA1 antibody can be used for detection of DDA1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1401 - Mouse Heart Tissue Lysate

psi-iconProperties
PURIFICATION:DDA Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DDA Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DDA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:DDA1
ALTERNATE NAMES:DDA Antibody: PCIA1, C19orf58, PCIA1, DET1- and DDB1-associated protein 1, Placenta cross-immune reaction antigen 1, PCIA-1
ACCESSION NO.:NP_003253
PROTEIN GI NO.:13129016
GENE ID:79016
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:DDA Antibody: DDA1 (DET1 and DDB1 associated 1), along with DET1 and DDB1 and a member of the UBE2E group of canonical ubiquitin-conjugating enzymes, comprise DDD-E2 complexes, which interact with multiple ubiquitin E3 ligases. One of these E3 ligases is Cul4-containing E3 ligase complex CRL4. Cells depleted of DDA1 spontaneously accumulate double-stranded DNA breaks in a similar fashion as Cul4A-, Cul4B-, or WDR23-depleted cells, suggesting that DDA1 interacts with the CRL4 complex and may be involved in the ubiquitination and subsequent proteasomal degradation of target proteins.
REFERENCES:1) Pick E, Lau O, Tsuge T, et al. Mammalian DET1 regulates Cul4A activity and forms stable complexes with E2 ubiquitin-conjugating enzymes. Mol. Cell. Biol. 2007; 27:4708-19.
2) Olma MH, Roy M, Le Bihan T, et al. An interaction network of the mammalian COP9 signalsome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. J. Cell Sci. 2009; 122:1035-44

ANTIBODIES FOR RESEARCH USE ONLY.

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