Datasheet

DcR1 Antibody
CATALOG NUMBER: 2299

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:DcR1 antibody can be used for detection of DcR1 by Western blot at 1 μg/mL. An approximate 65 kDa band can be detected. Antibody can also be used for immunoflourescence starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human, mouse, and rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 1404 - Mouse Liver Tissue Lysate
 3) Cat. No. 1464 - Rat Liver Tissue Lysate
PREDICTED MOLECULAR WEIGHT:65 kDa
IMMUNOGEN:DcR1 antibody was raised against a peptide corresponding to amino acids in the extracellular domain of human DcR1 precursor.

The immunogen is located within amino acids 90 - 140 of DcR1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DcR1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DcR1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:DcR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DcR1 Antibody: LIT, DCR1, TRID, CD263, TRAILR3, TRAIL-R3, DCR1-TNFR, LIT, UNQ321/PRO366, Tumor necrosis factor receptor superfamily member 10C, Decoy TRAIL receptor without death domain, DcR1
ACCESSION NO.:AF012536
PROTEIN GI NO.:2338421
OFFICIAL SYMBOL:TNFRSF10C
GENE ID:8794

Background

BACKGROUND:DcR1 Antibody: Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL. Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts are expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.
REFERENCES: 1) Pan G; O'Rourke K; Chinnaiyan et al.. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113
2) Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8
3) Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21
4) Degli-Esposti MA, Smolak PJ, Walczak H, et al, Smith CA. Cloning and characterization of TRAIL-R3, a novel member of the emerging TRAIL receptor family. J Exp Med 1997;186(7):1165-70

For Research Use Only