Datasheet

DCP2 Antibody
CATALOG NUMBER: 7049

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:DCP2 antibody can be used for detection of DCP2 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
PREDICTED MOLECULAR WEIGHT:46 kDa
SPECIFICITY:Multiple isoforms of DCP2 are known to exist.
IMMUNOGEN:DCP2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DCP2.

The immunogen is located within amino acids 340 - 390 of DCP2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DCP2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DCP2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DCP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DCP2 Antibody: NUDT20, NUDT20, m7GpppN-mRNA hydrolase, Nucleoside diphosphate-linked moiety X motif 20, Nudix motif 20
ACCESSION NO.:NP_689837
PROTEIN GI NO.:31542498
OFFICIAL SYMBOL:DCP2
GENE ID:167227

Background

BACKGROUND:DCP2 Antibody: The removal, or decapping, of eukaryotic mRNA is an important step in the degradation of mRNA. Decapping protein 2 (DCP2) is the major mRNA decapping enzyme in cells. It is a member of the Nudix hydrolases superfamily of proteins that predominantly catalyze the hydrolysis of small nucleoside diphosphate substrates linked to another moiety. DCP2 is widely expressed in multiple tissues at varying levels, with highest expression seen in testis and brain.
REFERENCES: 1) Song MG, Li Y, and Kiledjian M. Multiple mRNA decapping enzymes in mammalian cells. Mol. Cell 2010; 40:423-32.
2) Dunckley T and Parker R. The DCP2 protein is required for mRNA decapping in Saccharomyces cerevisiae and contains a functional MutT motif. EMBO J. 18:5411-22.
3) Bessman MJ, Frick DN, and O’Handley SF. The MutT proteins of “Nudix" hydrolases, a family of versatile, widely distributed, “housecleaning" enzymes. J. Biol. Chem. 1996; 271:25059-62

For Research Use Only