Datasheet

DCNP1 Antibody
CATALOG NUMBER: 7769

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:DCNP1 antibody can be used for detection of DCNP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 27 kDa

Observed: 24 kDa
SPECIFICITY:DCNP1 antibody is human, mouse and rat reactive.
IMMUNOGEN:DCNP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human DCNP1.

The immunogen is located within the first 50 amino acids of DCNP1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DCNP1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DCNP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DCNP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DCNP1 Antibody: DCNP1, C5orf20, Dendritic cell nuclear protein 1
ACCESSION NO.:NP_570900
PROTEIN GI NO.:18677770
OFFICIAL SYMBOL:C5orf20
GENE ID:140947

Background

BACKGROUND:The DCNP1 (dendritic cell nuclear protein 1) gene is specifically expressed in dendritic cells, potent antigen-presenting cells involved in activating naive T cells to initiate antigen-specific immune response (1). It is localized mainly in the perinucleus. One of the alleles (A/T) of this gene causes premature translation termination at aa 117 and has been associated with an increased prevalence of major depression in humans (2,3). DCNP1 may play a role in the pathogenesis of depressive disorders by enhancing corticotropin-releasing hormone expression in the hypothalamic paraventricular nucleus (4).
REFERENCES: 1) Masuda M, Senju S, Fujii Si S, et al. Identification and immunocytochemical analysis of DCNP1, a dendritic cell-associated nuclear protein. Biochem. Biophys. Res. Commun. 2002; 290:1022-9.
2) Willis-Owen SA, Shifman S, Copley RR, et al. DCNP1: a novel candidate gene for major depression. Mol. Psychiatry 2006; 11:121-2.
3) Bosker FJ, Hartman CA, Nolte IM, et al. Poor replication of candidate genes for major depressive disorder using genome-wide association data. Mol. Psychiatry 2011; 16:516-32.
4) Zhou T, Wang S, Ren H, et al. Dendritic cell nuclear protein-1, a novel depression-related protein, upregulates corticotropin-releasing hormone expression. Brain 2010; 133:3069-79.

For Research Use Only