Datasheet

DBX2 Antibody
CATALOG NUMBER: 6873

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:DBX2 antibody can be used for detection of DBX2 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1305 - Human Kidney Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
SPECIFICITY:DBX2 antibody is human specific. At least two isoforms of DBX2 are known to exist; this antibody will only detect the shortest isoform.
IMMUNOGEN:DBX2 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human DBX2.

The immunogen is located within amino acids 30 - 80 of DBX2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DBX2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DBX2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DBX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DBX2 Antibody: Homeobox protein DBX2, Developing brain homeobox protein 2
ACCESSION NO.:NP_001004329
PROTEIN GI NO.:116174748
OFFICIAL SYMBOL:DBX2
GENE ID:440097

Background

BACKGROUND:DBX2 Antibody: DBX2 is a member of the developing brain homeobox (DBX) protein family, but while the related protein DBX1 is expressed in various regions of the developing brain, DBX2 shows a more restricted pattern of expression in the brain, and is also expressed in some mesenchymal cells such as limb buds and tooth germs. It is thought that DBX1 and DBX2 promote the development of a subset of interneurons, some of which help mediate left-right coordination of locomotor activity. In Xenopus, DBX2 is involved in primary neurogenesis and early neural plate patterning, and is thought to act as a cross-repressive partner of NKX6-2 in the patterning of the ventral neural tube.
REFERENCES: 1) Shoji H, Ito T, Wakamatsu Y, et al. Regionalized expression of the Dbx family homeobox genes in the embryonic CNS of the mouse. Mech. Dev. 56:25-39.
2) Lacin H, Zhu Y, Wilson BA, et al. Dbx mediates neuronal specification and differentiation through cross-repressive, lineage-specific interactions with eve and hb9. Development 2009; 136:3257-66.
3) Ma P, Zhao S, Zeng W, et al. Xenopus Dbx2 is involved in primary neurogenesis and early neural plate patterning. Biochem. Biophys. Res. Commun. 2011; 412:170-4.

For Research Use Only