Datasheet

DBX1 Antibody
CATALOG NUMBER: 6455

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (87%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:DBX1 antibody can be used for detection of DBX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1405 - Mouse Kidney Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
SPECIFICITY:BX1 antibody is predicted to not cross-react with other DBX protein family members
IMMUNOGEN:DBX1 antibody was raised against a 15 amino acid synthetic peptide near the center of human DBX1.

The immunogen is located within amino acids 100 - 150 of DBX1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DBX1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DBX1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DBX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DBX1 Antibody: Homeobox protein DBX1, Developing brain homeobox protein 1
ACCESSION NO.:NP_001025036
PROTEIN GI NO.:304434796
OFFICIAL SYMBOL:DBX1
GENE ID:120237

Background

BACKGROUND:DBX1 Antibody: DBX1 is implicated in patterning the central nervous system during embryogenesis. DBX1 homeodomain transcription factor is expressed in progenitors which are critical in establishing cell fate allocation and cell diversity. DBX1 has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral interneurons, V0 and V1 neurons. DBX1-derived neurons are essential for the transgenic expression and generation of respiratory behavior both in vitro and in vivo.
REFERENCES: 1) Griveau A, Borello U, Causeret F, et al. A novel role for Dbx1-derived Cajal-Retzius cells in early regionalization of the cerebral cortical neuroepithelium. PLoS Biol. 2010; 8(7):e1000440
2) Causeret F, Ensini M, Teissier A, et al. Dbx1-expressing cells are necessary for the survival of the Mammalian anterior neural and craniofacial structures. PLoS One 2011; 6:e19367.
3) Pierani A, Moran-Rivard L, Sunshine MJ, et al. Control of interneuron fate in the developing spinal cord by the progenitor homeodomain protein Dbx1. Neuron 2001; 29:367-84
4) Gray PA, Hayes JA, Ling GY, et al. Developmental origin of preBotzinger complex respiratory neurons. J. Neurosci. 2010; 30:14883-95.

For Research Use Only