DARPP 32 (phospho Thr34) Antibody Cat. No.: XPS-1004

Availability: In stock

$0.00

 Price Options

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat, Xenopus
IMMUNOGEN: DARPP-32 (Thr34) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr34 of DARPP-32.
TESTED APPLICATIONS: WB
APPLICATIONS: Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Rabbit anti-DARPP-32 (Thr34) recognizes human, mouse, and rat forms of the protein. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
SPECIFICITY: DARPP 32 antibody is specific for the ~32k DARPP-32 phosphorylated at Thr34.
PREDICTED MOLECULAR WEIGHT: 32

psi-iconProperties
PURIFICATION:Affinity Purified
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
STORAGE CONDITIONS:For long term storage -80˚C is recommended, but shorter term storage at -20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚C.

psi-iconAdditional Info
OFFICIAL SYMBOL:Ppp1r1b
ACCESSION NO.:Q6J4I0
PROTEIN GI NO.:81884861
GENE ID:360616
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and ProzacĀ®.
REFERENCES:1) Svenningsson, P., Tzavara, E.T., Carruthers, R., Rachleff, I., Wattler, S., Nehls, M., McKinzie, D.L., Fienberg, A.A., Nomikos, G.G.and Greengard, P., "Diverse psychotomimetics act through a common signaling pathway," Science, 302 (2003) 1412 - 1415.
2) Lindskog, M., Svenningsson, P., Pozzi, L., Kim, Y., Fienberg, A.A., Bibb, J.A.., Fredholm, B.B., Nairn, A.C., Greengard, P. and Fisone, G., "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine, Nature (London), 418 (2002) 774 - 778.
3) Maldve, R.E., Zhang, T.A., Ferrani-Kile, K., Schreiber, S.S., Lippmann, M.J., Snyder, G.L., Feinberg, A.A., Leslie, S.W., Gonzales, R.A., and Morrisett, R.A., "DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens," Nature Neurosci. 5 (2002) 641 - 648.
4) Bibb, J.A., Snyder, G.L., Nishi, A., Yan, Z., Meijer, L., Fienberg, A.A., Tsai, L.H., Kwon, Y.T., Girault, J.A., Czernik, A.J., Huganir, R.L., Hemmings, Jr., H.C., Nairn, A.C., and Greengard, P., "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons, Nature (London) 402 (1999) 669 - 671.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.