Datasheet

DAPK2 Antibody
CATALOG NUMBER: 2323

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:DAPK2 antibody can be used for detection of DAPK2 by Western blot at 1 μg/mL. An approximately 42 kDa band can be detected. DAPK2 has no cross responses to DAPK1. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.

Antibody validated: Western Blot in human, mouse, and rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1202 - A431 Cell Lysate
 2) Cat. No. 1406 - Mouse Spleen Tissue Lysate
 3) Cat. No. 1465 - Rat Kidney Tissue Lysate
PREDICTED MOLECULAR WEIGHT:42 kDa
SPECIFICITY:DAPK2 has no cross responses to DAPK1.
IMMUNOGEN:DAPK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2.

This antigenic peptide is identical to the corresponding amino acids of mouse origin (1,2).

The immunogen is located within the last 50 amino acids of DAPK2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DAPK2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DAPK2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:DAPK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DAPK2 Antibody: DRP1, DRP-1, Death-associated protein kinase 2, DAP-kinase-related protein 1, DAP kinase 2
ACCESSION NO.:BAA88063
PROTEIN GI NO.:6521210
OFFICIAL SYMBOL:DAPK2
GENE ID:23604

Background

BACKGROUND:DAPK2 Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNγ induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.
REFERENCES: 1) Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ, Jenkins NA, Akira S. Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity. Oncogene 1999;18(23):3471-80
2) Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A. Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis. Mol Cell Biol 2000;20(3):1044-54 (WD0101)

For Research Use Only