Datasheet

DAP Antibody
CATALOG NUMBER: 7389

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (89%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1308 - Human Small Intestine Tissue Lysate
 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 11 kDa

Observed: 11 kDa
SPECIFICITY:DAP antibody is human, mouse and rat reactive.
IMMUNOGEN:DAP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DAP.

The immunogen is located within the last 50 amino acids of DAP.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DAP Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DAP Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DAP Antibody: DAP1, Death-associated protein 1, DAP-1
ACCESSION NO.:NP_004385
PROTEIN GI NO.:4758120
OFFICIAL SYMBOL:DAP
GENE ID:1611

Background

BACKGROUND:DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).
REFERENCES: 1) Deiss LP, Feinstein E, Berissi H, et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev. 1995; 9:15-30.
2) Koren I, Reem E, and Kimchi A. DAP1, a novel substrate of mTOR, negatively regulates autophagy. Curr. Biol. 2010; 20:1093-8.

For Research Use Only