DAF Antibody Cat. No.: 7937

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF.

The immunogen is located within amino acids 360 - 410 of DAF.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.
POSITIVE CONTROL:1) Cat. No. 1203 - A549 Cell Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
 3) Cat. No. 1201 – HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 48 kDa

Observed: 49 kDa

psi-iconProperties
PURIFICATION:DAF antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DAF antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DAF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:CD55
ALTERNATE NAMES:Decay-accelerating factor, CD55 molecule decay accelerating factor for complement (Cromer blood group), CD55, CR, TC, CROM
ACCESSION NO.:NP_001108224
PROTEIN GI NO.:168693643
GENE ID:1604
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The decay-accelerating factor (DAF), also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1). DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2). Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3).
REFERENCES:1) Nicholson-Weller A, March JP, Rosen CE, et al. Surface membrane expression by human blood leukocytes and platelts of decay-accelerating factor, a regulatory protein of the complement system. Blood 1985; 65:1237-44.
2) Seya T and Atkinson JP. Functional properties of membrane cofactor protein of complement. Biochem. J. 1989; 64:581-8.
3) Storry JR, Reid ME, and Yazer MH. The Cromer blood group system: a review. Immunohematology 2010; 109-18.

ANTIBODIES FOR RESEARCH USE ONLY.

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