Dact2 Antibody Cat. No.: 4859

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Dact2 antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human DACT2.

The immunogen is located within amino acids 150 - 200 of Dact2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: Dact2 antibody can be used for detection of Dact2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide

psi-iconProperties
PURIFICATION:Dact2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Dact2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Dact2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:DACT2
ALTERNATE NAMES:Dact2 Antibody: DPR2, DAPPER2, C6orf116, bA503C24.7, PP13671, Dapper homolog 2, Dapper antagonist of catenin 2
ACCESSION NO.:NP_999627
PROTEIN GI NO.:163965434
GENE ID:168002
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Dact2 Antibody: The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact2, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact2 is most prominent during the development of the thymus kidneys, and salivary gland. Dact2 is thought to play a role distinct from that of Dact1 with Dact2 having a greater impact on a beta-catenin-independent process termed planar cell polarity/convergent-extension signaling. Furthermore, Dact2 but not Dact1 can inhibit Nodal signaling by promoting the endocytic degradation of TGF-beta receptors. At least two isoforms of Dact2 are known to exist.
REFERENCES:1) Shimigori T, VanSant J, Paik E, et al. Members of the Wnt, Fz, and Frp gene families expressed in postnatal mouse cerebral cortex. J. Comp. Neurol.2004; 473:496-510.
2) Cheyette BNR, Waxman JS, Miller JR, et al. Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Dev. Cell2002; 2:449-61.
3) Katoh M and Katoh M. Identification and characterization of human DAPPER1 and DAPPER2 genes in silico. Int. J. Oncol.22:907-13.
4) Waxman JS, Hocking AM, Stoick CL, et al. Zebrafish DAPPER1 and DAPPER2 play distinct roles in Wnt-mediated developmental processes. Development2004; 131:5909-21.

ANTIBODIES FOR RESEARCH USE ONLY.

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