Cytochrome c Antibody [SJL2-4] Cat. No.: XA-1006

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Goat, Human, Monkey, Mouse, Rat, Sheep
IMMUNOGEN: Cytochrome C (monoclonal SJL2-4) antibody was raised against Horse cytochrome c.
TESTED APPLICATIONS: WB
APPLICATIONS: Cytochrome C antibody can be used for detection of horse cytochrome c by western blot. This product also recognizes human, mouse, rat, sheep, goat and monkey apocytochrome c. Method: Cell extracts from 293T cells (2x106) were separated into cytoplasmic and nuclear fraction, resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-cytochrome c antibody SJL2-4 at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.

psi-iconProperties
PURIFICATION:Cytochrome C (monoclonal SJL2-4) antibody was purified to ≥95% in SDS-PAGE.
CLONALITY:Monoclonal
ISOTYPE:IgG2a
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS containing 0.02% sodium azide
STORAGE CONDITIONS:Cytochrome c antibody can be stored at 4˚C, stable for one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:CYCS
ALTERNATE NAMES:CYC, Cytochrome c
ACCESSION NO.:P00004
PROTEIN GI NO.:119388048
GENE ID:100053958
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
REFERENCES:1) Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997).
2) Cytochrome c: can't live with it-can't live without it: J.C. Reed; Cell 91, 559 (1997).
3) Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked: J. Yang, et al.; Science 275, 1129 (1997).
4) Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997).

ANTIBODIES FOR RESEARCH USE ONLY.

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