Datasheet

Cyclin O Antibody
CATALOG NUMBER: 3859

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:Cyclin O antibody can be used for the detection of Cyclin O by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1410 - Mouse Bladder Tissue Lysate
PREDICTED MOLECULAR WEIGHT:39 kDa
SPECIFICITY:At least two isoforms of Cyclin O are known to exist; this antibody will recognize both isoforms.
IMMUNOGEN:Cyclin O antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Cyclin O.

The immunogen is located within the first 50 amino acids of Cyclin O.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Cyclin O Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Cyclin O Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Cyclin O antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Cyclin O Antibody: CCNU, UDG2, Cyclin-O
ACCESSION NO.:NP_066970
PROTEIN GI NO.:153791755
OFFICIAL SYMBOL:CCNO
GENE ID:10309

Background

BACKGROUND:Cyclin O Antibody: Cyclin O, also known as CCNO, has recently been identified as a Cdk1- and Cdk2-activating cyclin specific to apoptosis in lymphoid cells. Cyclin O binds to and activates Cdk2 in response to instrinsic apoptotic stimuli such as glucocorticoids or DNA-damaging agents. Supression of Cyclin O expression by shRNA leads to the inhibition of glucocorticoid and DNA-damage-induced apoptosis due to a failure of apical caspase activation while leaving the CD95 death receptor-mediated apoptosis intact. Note: this gene, which had a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase. A later publication identified this gene's product as a cyclin protein family member.
REFERENCES: 1) Roig MB, Roset R, Ortet L, et al. Identification of a novel cyclin required for the intrinsic apoptosis pathway in lymphoid cells. Cell Death Diff. 2009; 16:230-43.
2) Muller SJ and Caradonna S. Isolation and characterization of a human cDNA encoding uracil-DNA glycosylase. Biochim. Biophys. Acta 1991; 1088:197-207.
3) Muller SJ and Caradonna S. Cell cycle regulation of a human cyclin-like gene encoding uracil-DNA glycosylase. J. Biol. Chem. 1993; 268:1310-9

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