Datasheet

CXCR4-Lo Antibody
CATALOG NUMBER: 4443

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:CXCR4-Lo antibody can be used for Western blot at 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
SPECIFICITY:This antibody is specific for the longer isoform of CXCR4.
IMMUNOGEN:CXCR4-Lo antibody was raised against a peptide corresponding to nine amino acids near the amino terminus of human CXCR4 isoform a.

The immunogen is located within the first 50 amino acids of CXCR4-Lo.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CXCR4-Lo Antibody is purified IgG.
PHYSICAL STATE:Liquid
BUFFER:CXCR4-Lo Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CXCR4-Lo antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CXCR4-Lo Antibody: FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LESTR, NPY3R, NPYRL, HSY3RR, NPYY3R, D2S201E, C-X-C chemokine receptor type 4, FB22, CXC-R4
ACCESSION NO.:NP_001008540
PROTEIN GI NO.:56790927
OFFICIAL SYMBOL:CXCR4
GENE ID:7852

Background

BACKGROUND:CXCR4-Lo Antibody: Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The alpha-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.
REFERENCES: 1) Dimitrov DS. Cell1997; 91:721-30.
2) Feng Y et al. Science1996; 272:872-7.
3) Berson JF et al. J. Virol.1996; 70:6288-95.
4) Doranz BJ et al. Cell 1996; 85:1149-58.

For Research Use Only