Datasheet

CX3CR1 Antibody
CATALOG NUMBER: 2201

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (100%), Rabbit: (93%), Mouse: (93%), Bovine: (86%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CX3CR1 antibody can be used for detection of CX3CR1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1208 - THP-1 Cell Lysate
 2) Cat. No. 1212 - 3T3 Cell Lysate
 3) Cat. No. 17-008 - THP-1 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 39, 43 kDa

Observed: 50 kDa
SPECIFICITY:At least two isoforms of CX3CR1 are known to exist.
IMMUNOGEN:CX3CR1 antibody was raised against a peptide corresponding to amino acids in a extracellular loop of human CX3CR1.

The immunogen is located within amino acids 160 - 210 of CX3CR1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CX3CR1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CX3CR1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CX3CR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CX3CR1 Antibody: V28, CCRL1, GPR13, CMKDR1, GPRV28, CMKBRL1, CX3C chemokine receptor 1, Beta chemokine receptor-like 1, C-X3-C CKR-1
ACCESSION NO.:NP_001328
PROTEIN GI NO.:4503171
OFFICIAL SYMBOL:CX3CR1
GENE ID:1524

Background

BACKGROUND:CX3CR1 Antibody: CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was initially identified as the receptor for the transmembrane molecule fractalkine, and renamed CX3CR1. CX3CR1 is known to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.
REFERENCES: 1) Raport CJ, Schweickart VL, Eddy RL JR, et al. The orphan G-protein-coupled receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues. Gene 1995; 163:295-9
2) Combadiere C, Ahuja SK , and Murphy PM. Cloning, chromosomal localization, and RNA expression of a human β chemokine receptor-like gene. DNA Cell Biol. 1995;14:673-80
3) Combadiere C, Gao J, Tiffany HL, et al. Gene cloning, RNA distribution, and functional expression of mCX3CR1, a mouse chemotactic receptor for the CX3C chemokine fractalkine. Biochem. Biophys. Res. Commun. 1998; 253:728-32
4) Harrison JK, Barber CM, and Lynch KR. cDNA cloning of a G-protein-coupled receptor expressed in rat spinal cord and brain related to chemokine receptors. Neurosci. Lett. 1994; 169:85-9

For Research Use Only