CX3CR1 Antibody Cat. No.: 2093

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HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (82%)
IMMUNOGEN: Anti-CX3CR1 antibody (2093) was raised against a peptide corresponding to 20 amino acids near the amino terminus of mature human CX3CR1.

The immunogen is located within the first 50 amino acids of CX3CR1.
APPLICATIONS: WB: 1 -2 μg/mL; IF: 10-20 μg/mL, IHC-P: 2 μg/mL: Flow: 0.1 μg/mL .

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in rat samples; Immunofluorescence in human, mouse and rat samples; Flow cytometry in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
 3) Cat. No. 1466 - Rat Spleen Tissue Lysate
 4) Cat. No. 1208 - THP-1 Cell Lysate

Observed: 50 kD
psi-iconAdvanced Validation

KD Validation (Figure 2): Anti-CX3CR1 antibody (2093) specificity was further verified by CX3CR1 specific knockdown. CX3CR1 signal in 293 cells transfected with CX3CR1 siRNAs was disrupted in comparison with that in cells transfected with control siRNAs.

Regulated expression validation (Figure 13): CX3CR1 expression detected by anit-CX3CR1 antibodies (2093) was up-regulated by IL-2, which was inhibited by IL-15.

Deficiency Validation (Figure 15): CX3CR1+ cells detected by anti-CX3CR1 antibodies is localized in vascular wall of CX3CR1+/gfp mice, but not in CX3CR1gfp/gfp mice, where cells remained in perivascular region.

ISOFORMS: Human CX3CR1 has 3 isoforms, including isoform 1 (355aa, 40kD), isoform 2 (387aa, 44kD), isoform 3 (362aa, 41kD). Mouse CX3CR1 has one isoform (354aa, 40kD) and rat CX3CR1 also has one isoform (354aa, 40kD). 2093 can detect all isoforms of human, mouse and rat.

PURIFICATION:CX3CR1 Antibody is affinity chromatography purified via peptide column.
BUFFER:CX3CR1 Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:CX3CR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
ALTERNATE NAMES:CX3CR1 Antibody: V28, CCRL1, GPR13, CMKDR1, GPRV28, CMKBRL1, CX3C chemokine receptor 1, Beta chemokine receptor-like 1, C-X3-C CKR-1
PROTEIN GI NO.:4503171
GENE ID:1524
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CX3CR1 Antibody: CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, murine, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.
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