Why do we pre-bleed each animal?

The pre-bleeds are used as negative controls in different experiments and assays, such as ELISA testing. They can also be used to pre-select the animals with the lowest endogenous background for specific projects, such as generating polyclonal antibodies against targets like plant proteins

How much blood is taken for the pre-bleed?

Different animals are suject to different volumes of blood drawn for each bleed based on the size of the animal. These volumes are listed below:

  • Rabbit: ~5.0mL
  • Mouse: ~0.05mL
  • Rat: ~0.5mL
  • Giunea Pig: ~0.5mL
  • Chicken: ~1.0mL
  • Goat: ~25mL
  • Llama: ~25mL