Datasheet

CUEDC1 Antibody
CATALOG NUMBER: 4829

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CUEDC1 antibody can be used for detection of CUEDC1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
 2) Cat. No. 10-201 - Human Liver Tissue Slide
SPECIFICITY:CUEDC1 antibody will not cross-react with CUEDC2.
IMMUNOGEN:CUEDC1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human CUEDC1.

The immunogen is located within amino acids 310 - 360 of CUEDC1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CUEDC1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CUEDC1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CUEDC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CUEDC1 Antibody: CUE domain-containing protein 1
ACCESSION NO.:AAH56882
PROTEIN GI NO.:34785153
OFFICIAL SYMBOL:CUEDC1
GENE ID:404093

Background

BACKGROUND:CUEDC1 Antibody: The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, ~40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. CUE domains are present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found. CUEDC1 (CUE domain-containing protein 1) is a 386 amino acid protein that contains one CUE domain, suggesting a possible role in protein trafficking and degradation pathways. Two isoforms of CUEDC1 exist due to alternative splicing events. Defects in the gene encoding CUEDC1 may be associated with early stage cervical cancer, implicating CUEDC1 as a potential tumor marker.
REFERENCES: 1) Shih SC, Prag G, Francis SA, et al. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J.2003; 22:1273-81.
2) Ponting CP. Proteins of the endoplasmic-reticulum-associated degradation pathway: domain detection and function prediction. Biochem. J.2000; 351:527-35.
3) Prag G, Misra S, Jones EA, et al. Mechanism of ubiquitin recognition by the CUE domain of Vps9p. Cell2003; 113:609-20.
4) Kang RS, Daniels CM, Francis SA, et al. Solution structure of a CUE-ubiquitin complex reveals a conserved mode of ubiquitin binding. Cell2003; 113:621-30.

For Research Use Only