CTRP3 Antibody Cat. No.: 3565

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Anti-CTRP3 antibody (3565) was raised against a peptide corresponding to 16 amino acids near the center of human CTRP3.

The immunogen is located within amino acids 90-140 of CTRP3.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 1 μg/mL; IHC: 2 μg/mL; IF: 10 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: Multiple isoforms of CTRP3 are known to exist. CTRP3 antibody is predicted to not recognize other members of the CTRP protein family.
POSITIVE CONTROL:1) Cat. No. 1401 - Mouse Heart Tissue Lysate
 2) Cat. No. 1203 - A549 Cell Lysate
 3) Cat. No. 1205 - Jurkat Cell Lysate
 4) Cat. No. 1282 - 3T3/NIH Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 27kD

Observed: 30 kD (Post-modyfication: 1 N-linked glycosylation)
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 2): Anti-CTRP3 antibodies (3565) detected human CTRP3 recombinant protein.

ISOFORMS: Human CTRP3 has 3 isoforms, including isoform 1 (246aa, 27kD), isoform 2 (198aa, 22kD) and isoform 3 (319aa, 35kD). Mouse CTRP3 has one isoform (246aa, 27kD) and Rat CTRP3 also has one isoform (319aa, 35kD). 3565 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:CTRP3 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:CTRP3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CTRP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:C1QTNF3
ALTERNATE NAMES:CTRP3 Antibody: CORS, CORCS, CTRP3, CORS26, C1ATNF3, CORS-26, UNQ753/PRO1484, Complement C1q tumor necrosis factor-related protein 3, Collagenous repeat-containing sequence 26 kDa protein
ACCESSION NO.:NP_852100
PROTEIN GI NO.:62739183
GENE ID:114899
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CTRP3 Antibody: Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. It has been suggested that CTRP3 may play a role in skeletal development.
REFERENCES:1) Fantuzzi. J. Allergy Clin. Immunol. 2005; 115:911-9.
2) Tsao et al. Euro. J. Pharmacol. 2002; 440:213-21.
3) Wong et al. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
4) Shapiro and Scherer. Curr. Biol. 1998; 8:335-8.

ANTIBODIES FOR RESEARCH USE ONLY.

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