Datasheet

CTRP1 Antibody
CATALOG NUMBER: 3557

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Rat
TESTED APPLICATIONS:IF, IHC-P
APPLICATIONS:CTRP1 antibody can be used for the detection of CTRP1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1305 - Human Kidney Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
SPECIFICITY:These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size.
IMMUNOGEN:CTRP1 (NT) antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human CTRP1.

The immunogen is located within amino acids 40 - 90 of CTRP1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CTRP1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CTRP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CTRP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CTRP1 Antibody: GIP, CTRP1, ZSIG37, UNQ310/PRO353, Complement C1q tumor necrosis factor-related protein 1, G protein-coupled receptor-interacting protein, GIP
ACCESSION NO.:NP_940995
PROTEIN GI NO.:38372917
OFFICIAL SYMBOL:C1QTNF1
GENE ID:114897

Background

BACKGROUND:CTRP1 Antibody: Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. In obese (ob/ob) mice, RT-PCR analysis showed that mCTRP1 transcripts are seen at substantially higher levels in adipose tissues compared to those of normal mice.
REFERENCES: 1) Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.
2) Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.
3) Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
4) Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8.

For Research Use Only