CTLA 4 Recombinant Protein Cat. No.: 96-219

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psi-iconSpecifications
SPECIES:Human
SOURCE SPECIES:HEK293 cells
SEQUENCE:Ala 37 - Ser 160
FUSION TAG:C-Fc Tag
TESTED APPLICATIONS:WB
APPLICATIONS:This recombinant protein can be used for WB. For research use only.
BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein Fc Chimera at 5μg/mL (100μL/well) can bind rhB7-1/ CD80 Protein with a linear range of 0. 1 - 2μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein Fc Chimera at 5μg/ml (100μl/well) can bind rh PD-L1 /B7-H1 /CD274 Protein with a linear range of 1 - 10 ug/mL.
psi-iconProperties
PURITY:>98% as determined by SDS-PAGE.
PREDICTED MOLECULAR WEIGHT:40 kDa
PHYSICAL STATE:Lyophilized
BUFFER:Tris with Glycine, Arginine and NaCl, pH7.5
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12
ACCESSION NO.:NP_005205.2
OFFICIAL SYMBOL:CTLA4
GENE ID:1493
psi-iconBackground and References
BACKGROUND:CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
REFERENCES:1) Waterhouse P, et al., 1995, Science 270 (5238): 985–8.
2) Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596–602.
3) Rudd, CE. et al., 2009, Immunol. Rev. 229 (1): 12-26.

FOR RESEARCH USE ONLY.

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