| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| IMMUNOGEN: | CSN8 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human CSN8. The immunogen is located within the last 50 amino acids of CSN8. |
| TESTED APPLICATIONS: | ELISA, WB |
| APPLICATIONS: | CSN8 antibody can be used for detection of CSN8 by Western blot at 2 μg/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
Specifications| PURIFICATION: | CSN8 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | CSN8 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | CSN8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | COPS8 |
| ALTERNATE NAMES: | CSN8 Antibody: COP9, CSN8, SGN8, COP9 signalosome complex subunit 8, COP9 homolog |
| ACCESSION NO.: | NP_006701 |
| PROTEIN GI NO.: | 5729779 |
| GENE ID: | 10920 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | CSN8 Antibody: The COP9 signalosome (CSN) is an evolutionarily conserved protein complex of the eight subunits that interacts with deubiquitinating enzymes and protein kinases and is highly homologous to the lid sub-complex of 26S proteasome. The CSN complex is an essential regulator of the ubiquitin conjugation pathway by mediating the deneddylation of the SCF-type E3 ligase complexes, which leads to a decrease in ubiquitin ligase activity of SCF-comlpexes such as SCF, CSA or DDB2. It is also involved in phosphorylation of p53, c-jun/JUN, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN8 encodes the smallest and the least conserved but first identified subunit of CSN. Recent studies show CSN8 is essential for Drosophila development and is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence. |
| REFERENCES: | 1) Wei N and Deng XW. The COP9 signalosome. Annu. Rev. Cell Dev. Biol.2003; 19:261-86. | 2) Bech-Otschir D, Seeger M, and Dubiel W. The COP9 signalosome: at the interface between signal transduction and ubiquitin-dependent proteolysis. J. Cell Sci.2002; 115:467-73. | 3) Groisman R, Polanowska J, Kuraoka I, et al. The ubiquitin ligase activity in the DDB2 and regulated by the COP9 signalosome in response to DNA damage. Cell2003; 113:357-67. | 4) Uhle S, Medalia O, Waldron R, et al. Protein kinase CK2 and protein kinase D are associated with the COP9 signalosome. EMBO J.2003; 22:1302-12. |
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