Datasheet

CRTC2 Antibody
CATALOG NUMBER: 7325

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (94%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CRTC2 antibody can be used for detection of CRTC2 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1308 - Human Small Intestine Tissue Lysate
 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 67, 76 kDa

Observed: 70 kDa
SPECIFICITY:Multiple isoforms of CRTC2 are known to exist. CRTC2 antibody is predicted to not cross-react with CRTC1.
IMMUNOGEN:CRTC2 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRTC2.

The immunogen is located within the last 50 amino acids of CRTC2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CRTC2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CRTC2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CRTC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CRTC2 Antibody: TORC2, TORC-2, TORC2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2
ACCESSION NO.:NP_859066
PROTEIN GI NO.:32171215
OFFICIAL SYMBOL:CRTC2
GENE ID:200186

Background

BACKGROUND:CRTC2 Antibody: CRTC2, also known as TORC2 (Transducers Of Regulated cAMP Response Element-Binding (CREB)) 2 and the related protein CRTC1 are potent CREB coactivators that are exported from the nucleus in a CRM1-dependent manner via phosphorylation-dependent interactions. Studies suggest that their phosphorylation and nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP. CRTC2 is present in both B and T-lymphocytes and abundantly expressed in the thymus. Its activity is important in regulating the expression of genes involved in cellular energy metabolism while CRTC1 is essential for energy balance and fertility.
REFERENCES: 1) Conkright MD, Canettieri G, Screaton R, et al. TORCs: transducers of regulated CREB activity. Mol. Cell 2003; 12:413-23.
2) Iourgenko V, Zhang W, Mickanin C, et al. Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells. Proc. Natl. Acad. Sci. USA 2003; 100:12147-52.
3) Bittinger MA, McWhinnie E, Meltzer J, et al. Activation of cAMP response element-mediated gene expression by regulated nuclear transport of TORC proteins. Curr. Biol. 2004; 14: 2156-61.
4) Jiang S, Inada T, Tanaka M, et al. Involvement of TORC2, a CREB co-activator, in the in vivo-specific transcriptional control of HTLV-1. Retrovirology 2009; 6:73.

For Research Use Only