Datasheet

CRMP1 Antibody
CATALOG NUMBER: 3625

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (93%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CRMP1 antibody can be used for the detection of CRMP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1223 - Caco-2 Cell Lysate
 2) Cat. No. 1303 - Human Brain Tissue Lysate
 3) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 63, 75 kDa

Observed: 63, 75 kDa
IMMUNOGEN:CRMP1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human CRMP1.

The immunogen is located within amino acids 290 - 340 of CRMP1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CRMP1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CRMP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CRMP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CRMP1 Antibody: DRP1, DRP-1, CRMP-1, DPYSL1, ULIP-3, ULIP3, Dihydropyrimidinase-related protein 1, Collapsin response mediator protein 1
ACCESSION NO.:AAH07613
PROTEIN GI NO.:14043246
OFFICIAL SYMBOL:CRMP1
GENE ID:1400

Background

BACKGROUND:CRMP1 Antibody: Collapsin-response mediator proteins (CRMPs) are highly expressed in the developing brain where they play major roles in axonal outgrowth, neurite differentiation, and apoptosis. Their continued expression in areas of high synaptic remodeling such as the cerebellum, hippocampus, and the olfactory system suggests that these proteins may also be involved in adult brain plasticity. CRMP-1 was initially identified as a dihydro-pyrimidinase expressed exclusively in brain; later studies have shown that it is involved with neurotrophin (NT) 3-induced neurite formation and outgrowth. CRMP-1 localization switches from axonal to somatodendritic when neurons reach functional maturity, suggesting that it is involved in early neuronal differentiation as well as in later processes related to the survival or death of the newly generated neurons.
REFERENCES: 1) Charrier E, Reibel S, Rogemond V, et al. Collapsin response mediator proteins (CRMPs): involvement in nervous system development and adult neurodegenerative disorders. Mol. Neurobiol. 2003; 28:51-64.
2) Cameron HA and McKay RD. Adult neurogenesis produces a large pool of new granule cells in the dentate gyrus.J. Comp. Neurol. 2001; 435:406-417.
3) Hamajima N, Matsuda K, Sakata S, et al. A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution. Gene. 1996; 180:157-63.
4) Quach TT, Duchemin A-M, Rogemond V, et al. Involvement of collapsin response mediator proteins in the neurite extension induced by neurotrophins in dorsal root ganglion neurons. Mol. Cell. Neurosci. 2004; 25:433-43.

For Research Use Only