Datasheet

CRISP2 Antibody
CATALOG NUMBER: 5873

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CRISP2 antibody can be used for detection of CRISP2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
IMMUNOGEN:CRISP2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CRISP2.

The immunogen is located within the first 50 amino acids of CRISP2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CRISP2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CRISP2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CRISP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CRISP2 Antibody: CT36, TPX1, TSP1, GAPDL5, CRISP-2, Cysteine-rich secretory protein 2, Testis-specific protein TPX-1
ACCESSION NO.:NP_001135880
PROTEIN GI NO.:215422434
OFFICIAL SYMBOL:CRISP2
GENE ID:7180

Background

BACKGROUND:CRISP2 Antibody: The cysteine-rich secretory proteins (CRISP) family is a group of four proteins that are strongly expressed in the male reproductive tract and have been implicated in having roles in male fertility. CRISP2, also known as TPX1, has been implicated in the adhesion between spermatids and Sertoli cells, and with CRISP1, is thought to be involved in sperm-egg fusion. CRISP2 has been shown to regulate the Ca2+ influx through ryanodine receptors (RYR) and may influence the acrosome reaction or sperm motility. CRISP2 has also been shown to bind to the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) and localizes to the developing acrosome, suggesting this CRISP2-MAP3K11 complex may have a role in acrosome development.
REFERENCES: 1) Cohen DJ, Busso D, Da Ros V, et al. Participation of cysteine-rich secretory proteins (CRISP) in mammalian sperm-egg interaction. Int. J. Dev. Biol.2008; 52:737-42.
2) Maeda T, Sakashita M, Ohba Y, et al. Molecular cloning of the rat Tpx-1 responsible for the interaction between spermatogenic and Sertoli cells. Biochem. Biophys. Res. Commun.1998; 248:140-6.
3) Busso D, Goldweic NM, Hayashi M, et al. Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion. Biol. Reprod.2007; 76:701-8.
4) Gibbs GM, Scanlon MJ, Swarbrick J, et al. The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. J. Biol. Chem.2006; 281:4156-63.

For Research Use Only