Datasheet

CRIM1 Antibody
CATALOG NUMBER: 6961

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (88%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:CRIM1 antibody can be used for detection of Crim1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 17-005 - Jurkat Cell Slide
SPECIFICITY:CRIM1 antibody is human reactive. CRIM1 antibody is predicted to not cross-react with CRIM2.
IMMUNOGEN:CRIM1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human Crim1.

The immunogen is located within amino acids 910 - 960 of CRIM1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CRIM1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CRIM1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CRIM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CRIM1 Antibody: S52, CRIM-1, S52, UNQ1886/PRO4330, Cysteine-rich motor neuron 1 protein, Cysteine-rich repeat-containing protein S52
ACCESSION NO.:NP_057525
PROTEIN GI NO.:10092639
OFFICIAL SYMBOL:CRIM1
GENE ID:51232

Background

BACKGROUND:CRIM1 Antibody: CRIM1 (cysteine-rich motor neuron 1), a glycosylated type I transmembrane protein, plays a role in tissue development i.e. capillary formation and maintenance during angiogenesis. It contains an N-terminal IGF-binding protein-like motif and six von Willebrand-like cysteine-rich repeats (CRRs) in its extracellular domain. CRIM1 interacts with BMP4 and BMP7 via the CRRs and functions as an antagonist. CRIM1 is developmentally expressed in a number of tissues including the pancreas, kidney, placenta, brain and blood vessels. CRIM1 may participate in CNS and placental development by interacting with growth factors involved in motor neuron differentiation and survival.
REFERENCES: 1) Kolle G, Georgas K, Holmes GP, et al. CRIM1, a novel gene encoding a cysteine-rich repeat protein, is developmentally regulated and implicated in vertebrate CNS development and organogenesis. Mech. Dev. 2000; 90:181-93.
2) Glienke J, Sturz A, Menrad A, et al. CRIM1 is involved in endothelial cell capillary formation in vitro and is expressed in blood vessels in vivo. Mech. Dev. 2002; 119:165-75.
3) Wilkinson L, Kolle G, Wen D, et al. CRIM1 regulates the rate of processing and delivery of bone morphogenetic proteins to the cell surface. J. Biol. Chem. 2003; 278:34181-8.
4) Pennisi DJ, Kinna G, Chiu HS, et al. Crim1 has an essential role in glycogen trophoblast cell and sinusoidal-trophoblast giant cell development in the placenta. Placenta 2012; 33:175-82.

For Research Use Only