Datasheet

CRH Antibody
CATALOG NUMBER: 7797

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CRH antibody can be used for detection of CRH by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1309 - Human Placenta Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 22 kDa

Observed: 29 kDa
SPECIFICITY:CRH antibody is human specific.
IMMUNOGEN:CRH antibody was raised against a 16 amino acid peptide near the amino terminus of human CRH.

The immunogen is located within amino acids 30 - 80 of CRH.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CRH antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CRH antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CRH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CRH Antibody: CRF, Corticoliberin, Corticotropin-releasing factor, CRF
ACCESSION NO.:NP_000747
PROTEIN GI NO.:4503041
OFFICIAL SYMBOL:CRH
GENE ID:1392

Background

BACKGROUND:Corticotropin-releasing hormone (CRH) is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress (1). In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta where it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturitio (2). Marked reduction in this protein has been observed in association with Alzheimer disease (3).
REFERENCES: 1) Maras PM and Baram TZ. Sculpting the hippocampus from within: stress, spines, and CRH. Trends Neurosci. 2012; 315-24.
2) Vrachnis N, Malamas FM, Sifakis S, et al. Immune aspects and myometrial actions of progesterone and CRH in labor. Clin. Dev. Immunol. 2012; 937618.
3) Leake A, Perry EK, Perry RH, et al. Neocortical concentrations of neuropeptides in senile dementia of the Alzheimer and Lewy body type: comparison with Parkinson’s disease and severity correlations. Biol. Psychiatry 1991; 29:357-64.

For Research Use Only