CRH Antibody Cat. No.: 7797

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: CRH antibody was raised against a 16 amino acid peptide near the amino terminus of human CRH.

The immunogen is located within amino acids 30 - 80 of CRH.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: CRH antibody can be used for detection of CRH by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: CRH antibody is human specific.
POSITIVE CONTROL:1) Cat. No. 1309 - Human Placenta Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 22 kDa

Observed: 29 kDa

psi-iconProperties
PURIFICATION:CRH antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:CRH antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CRH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:CRH
ALTERNATE NAMES:CRH Antibody: CRF, Corticoliberin, Corticotropin-releasing factor, CRF
ACCESSION NO.:NP_000747
PROTEIN GI NO.:4503041
GENE ID:1392
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Corticotropin-releasing hormone (CRH) is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress (1). In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta where it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturitio (2). Marked reduction in this protein has been observed in association with Alzheimer disease (3).
REFERENCES:1) Maras PM and Baram TZ. Sculpting the hippocampus from within: stress, spines, and CRH. Trends Neurosci. 2012; 315-24.
2) Vrachnis N, Malamas FM, Sifakis S, et al. Immune aspects and myometrial actions of progesterone and CRH in labor. Clin. Dev. Immunol. 2012; 937618.
3) Leake A, Perry EK, Perry RH, et al. Neocortical concentrations of neuropeptides in senile dementia of the Alzheimer and Lewy body type: comparison with Parkinson’s disease and severity correlations. Biol. Psychiatry 1991; 29:357-64.

ANTIBODIES FOR RESEARCH USE ONLY.

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