CRF Receptor Antibody Cat. No.: 46-648

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: The immunogen for this antibody is: C-NEEKKSKVHYH
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59(3):485-92; PMID: 11179443) . Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunocytochemistry: This data if from a previous batch: http://www.biocompare.com/Product-Reviews/152450-Immunocytochemistry-for-CRH-receptor-type-1/
POSITIVE CONTROL:1) Cat. No. 1320 - Human Colon Tissue Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:CRHR1
ALTERNATE NAMES:CRHR1, CRF-R, corticotropin releasing hormone receptor 1, CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor
ACCESSION NO.:NP_004373.2
PROTEIN GI NO.:19923245
GENE ID:1394
psi-iconBackground and References
REFERENCES:1) Rissman RA, Lee KF, Vale W, Sawchenko PE. Corticotropin-releasing factor receptors differentially regulate stress-induced tau phosphorylation. J Neurosci. 2007 Jun 13;27(24):6552-62.

ANTIBODIES FOR RESEARCH USE ONLY.

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