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CRBN Antibody
CATALOG NUMBER: 8115

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (94%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 49 kDa

Observed: 54 kDa
SPECIFICITY:CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.
IMMUNOGEN:CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN.

The immunogen is located within the last 50 amino acids of CRBN.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CRBN antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CRBN antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CRBN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Cereblon, AD-006, DKFZp781K0715, MGC27358, MRT2A
ACCESSION NO.:NP_057386
PROTEIN GI NO.:39545580
OFFICIAL SYMBOL:CRBN
GENE ID:51185

Background

BACKGROUND:CRBN, a member of the Lon protease protein family, plays a role in brain development (1). It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2). CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3,4).
REFERENCES: 1) Xin W, Xiaohua N, Peilin C, et al. Primary function analysis of human mental retardation related gene CRBN. Mol. Biol. Rep. 2008; 35:251-6.
2) Gandhi AK, Kang J, Havens CG, et al. Immunomodulatory agents lenalidomide and pomalidomide co-stimulate T cells by inducing degradation of T cell repressors Ikaros and Aiolos via modulation of the E3 ubiquitin ligase complex CRL4(CRBN). Br. J. Haematol. 2014; 164:811-21.
3) Liu J, Ye J, Zou X, et al. CRL4A (CRBN) E3 ubiquitin ligase restricts BK channel activity and prevents epileptogenesis. Nat. Commun. 2014; 5:3924.
4) Heintel D, Rocci A, Ludwig H, et al. High expression of cereblon (CRBN) is associated with improved clinical response in patients with multiple myeloma treated with lenalidomide and dexamethasone. Br. J. Haematol. 2013; 161:695-700.

For Research Use Only