Datasheet

CRB1 Antibody
CATALOG NUMBER: 7153

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (82%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:CRB1 antibody can be used for detection of CRB1 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1308 - Human Small Intestine Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 142 kDa
SPECIFICITY:Alternatively spliced transcript variants have been observed. CRB1 antibody i spredicted to not cross-react with CRB2.
IMMUNOGEN:Rabbit polyclonal CRB1 antibody was raised against an 18 amino acid peptide near the center of human CRB1.

The immunogen is located within amino acids 780 - 830 of CRB1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CRB1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CRB1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CRB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CRB1 Antibody: LCA8, RP12, Protein crumbs homolog 1
ACCESSION NO.:NP_001180569
PROTEIN GI NO.:302370926
OFFICIAL SYMBOL:CRB1
GENE ID:23418

Background

BACKGROUND:CRB1 Antibody: CRB1 (Crumbs homolog 1) plays a role in photoreceptor morphogenesis in the retina. It may maintain cell polarization and adhesion. CRB1 is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. The first identified human homolog, CRB1, is expressed in retina and some parts of the brain, leaving room for another homolog to function in epithelial tissues. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis.
REFERENCES: 1) Van de Pavert SA, Kantardzhieva A, Malysheva A, et al. Crumbs homologue 1 is required for maintenance of photoreceptor cell polarization and adhesion during light exposure. J. Cell Sci. 2004; 117:4169-77.
2) Van de Pavert SA, Sanz AS, Aartsen WM, et al. Crb1 is a determinant of retinal apical Muller glia cell features. Glia 2007; 55:1486-97.
3) Den Hollander AI, Johnson K, de Kok YJ, et al. CRB1 has a cytoplasmic domain that is functionally conserved between human and Drosophila. Hum. Mol. Genet. 2001; 10:2767-73.
4) Bujakowska K, Audo I, Mohand-Said S, et al. CRB1 mutations in inherited retinal dystrophies. Hum. Mutat. 2012; 33:306-15.

For Research Use Only