CRB1 Antibody Cat. No.: 7153

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (82%)
IMMUNOGEN: Rabbit polyclonal CRB1 antibody was raised against an 18 amino acid peptide near the center of human CRB1.

The immunogen is located within amino acids 780 - 830 of CRB1.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: CRB1 antibody can be used for detection of CRB1 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
SPECIFICITY: Alternatively spliced transcript variants have been observed. CRB1 antibody i spredicted to not cross-react with CRB2.
POSITIVE CONTROL:1) Cat. No. 1308 - Human Small Intestine Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 142 kDa

psi-iconProperties
PURIFICATION:CRB1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:CRB1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CRB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:CRB1
ALTERNATE NAMES:CRB1 Antibody: LCA8, RP12, Protein crumbs homolog 1
ACCESSION NO.:NP_001180569
PROTEIN GI NO.:302370926
GENE ID:23418
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CRB1 Antibody: CRB1 (Crumbs homolog 1) plays a role in photoreceptor morphogenesis in the retina. It may maintain cell polarization and adhesion. CRB1 is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. The first identified human homolog, CRB1, is expressed in retina and some parts of the brain, leaving room for another homolog to function in epithelial tissues. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis.
REFERENCES:1) Van de Pavert SA, Kantardzhieva A, Malysheva A, et al. Crumbs homologue 1 is required for maintenance of photoreceptor cell polarization and adhesion during light exposure. J. Cell Sci. 2004; 117:4169-77.
2) Van de Pavert SA, Sanz AS, Aartsen WM, et al. Crb1 is a determinant of retinal apical Muller glia cell features. Glia 2007; 55:1486-97.
3) Den Hollander AI, Johnson K, de Kok YJ, et al. CRB1 has a cytoplasmic domain that is functionally conserved between human and Drosophila. Hum. Mol. Genet. 2001; 10:2767-73.
4) Bujakowska K, Audo I, Mohand-Said S, et al. CRB1 mutations in inherited retinal dystrophies. Hum. Mutat. 2012; 33:306-15.

ANTIBODIES FOR RESEARCH USE ONLY.

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