CPSF1 Antibody Cat. No.: 29-411

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CPSF1.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: CPSF1 antibody can be used for detection of CPSF1 by ELISA at 1:62500. CPSF1 antibody can be used for detection of CPSF1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
POSITIVE CONTROL:1) Tranfected 293T Cell Lysate
PREDICTED MOLECULAR WEIGHT: 161 kDa

psi-iconProperties
PURIFICATION:Antibody is purified by peptide affinity chromatography method.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:For short periods of storage (days) store at 4˚C. For longer periods of storage, store CPSF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:CPSF1
ALTERNATE NAMES:CPSF1, CPSF160, HSU37012, P/cl.18
ACCESSION NO.:NP_037423
PROTEIN GI NO.:56676371
GENE ID:29894
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CPSF1 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly (A) polymerase and other factors to bring about cleavage and poly (A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.
REFERENCES:1) Beausoleil, S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135.

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