SARS-CoV-2 (COVID-19) Antibody Pairs
ProSci Incorporated has launched matched SARS-CoV-2 (COVID-19, 2019-nCoV) antibody pairs validated by sandwich ELISA. These spike antibody pair and nucleocapsid antibody pairs are suitable for COVID-19 antigen test, ELISA, lateral flow and other immunoassay.
SARS-CoV-2 Nucleocapsid Matched Pair Antibodies
SARS-CoV-2 Spike Matched Pair Antibodies
|Pair||Capture Ab.||Host||Clonality||Detection Ab.||Host||Clonality||Target Protein||Binding Protein|
New Product Pipeline for SARS-CoV-2
ProSci Incorporated continues to develop more SARS-CoV-2 (COVID-19, 2019-nCoV) antibodies and proteins against the full proteome of SARS-CoV-2, including Envelop, Matrix, NSPs and ORFs, which will be launched soon.
SARS-CoV-2 (COVID-19) Research Reagents
ProSci Incorporated’s SARS-CoV-2 Spike Antibody (Cat. No. 3525) immediately met researchers’ needs at the outset of the COVID-19 pandemic and as a result is one of the most cited antibodies used in SARS-CoV-2 and COVID-19 research. ProSci now provides antibodies and recombinant proteins representing the entire SARS-CoV-2 proteome as well as for viral cellular entry factors ACE2, TMPRSS2, and Furin.
Multiple SARS-CoV-2 variants have evolved since the beginning of the pandemic several demonstrating greater transmissibility. In particular, the spread of Alpha variant (N501Y) as a Virus of Concern (VoC) with Spike protein mutation P681H expanded at such a rapid rate from February 2021 through May 2021 that incidence rose to 50% of worldwide COVID-19 cases. Delta has become a more recent VoC and is quickly displacing the Alpha variant. To better understand the role played by spike protein cleavage in SARS-CoV-2 biology and COVID-19 pathology, ProSci Incorporated is the first to offer an antibody specifically against the SARS-CoV-2 Alpha variant P681H mutation (Cat. No. 9359).
ProSci offers a comprehensive list of SARS-CoV-2 antibodies against viral proteins; spike, nucleocapsid, envelope, ORF, and NSP. These antibodies can be used with various applications: IHC, Flow Cytometry, ELISA, WB, and Lateral-Flow.
Diagram of SARS-CoV-2 with Validated Antibodies
|Target||SARS-CoV-2 Structure Protein Antibodies|
SARS-CoV-2 (COVID-19, 2019-nCoV) Recombinant Protein
Recombinant proteins developed in-house & tested with validated antibodies. Our recombinant proteins expressed are in various cell lines and expression systems for the novel coronavirus SARS-CoV-2 (COVID-19, 2019-nCoV): Full/partial length, tagged/untagged, and biologically active.
Diagram of SARS-CoV-2 Proteins
In addition to our coronavirus COVID-19 (SARS-CoV-2) antibodies & recombinant proteins, similar related reagents are available for SARS-CoV. The two viruses, SARS-CoV-2 and SARS-CoV, have approximately 79% sequence identity. For potential antibody cross reactivity refer to the table below.
Diagram of SARS-CoV-2 Proteins
|Product||Cat. No.||Host||Application||Clonality||Protein Structure||Identity||Homology||Blocking Peptide|
SARS-CoV Recombinant Protein
|Product||Cat. No.||Source||Fusion Tag||Sequence|
Ser 14 - Pro 1195
For COVID-19 (SARS-CoV-2) receptor reagents, check out our ACE2 products! In addition to all our COVID-19 related antibody reagents, we also offer custom antibody services for polyclonal, monoclonal, and single domain antibodies.
What is SARS-CoV-2?
SARS-CoV-2 (COVID-19, 2019-nCoV) is a major public heatlh threat with more than 1,200,000 deaths worldwide. The first stage in the coronavirus life cycle is viral entry where the viral spike protein will bind to a specific cell surface receptor. The spike proteins of SARS-CoV-2, as well as SARS-CoV-1 and NL63 use angiotensin-converting enzyme 2 (ACE2) as a receptor for binding. An antibody for this ACE2 protein has been produced by ProSci Inc. (Catalog No. 3217) that has been shown to function in immunohistochemistry and immunofluorescence experiments in human samples, and western blot experiments in human, mouse, and rat samples.
SARS-CoV-2 (COVID-19) Background
COVID-19 is an acute respiratory disease caused by novel coronavirus SARS-CoV-2 also known as 2019-nCoV. On March 11, 2020, the World Health Organization (WHO) characterized COVID-19 as a pandemic[i]. COVID-19 coronavirus SARS-CoV-2 belongs to the Betacoronavirus genus originating from bats. Betacoronaviruses can infect mammals, are zoonotic pathogens, and can cause severe respiratory disease in humans. Other viruses in this family are SARS coronavirus and MERS coronavirus. COVID-19 (SARS-CoV-2) has approximately 79% sequence identity to SARS-CoV and 50% to MERS-CoV.[ii] In addition, homology modeling shows COVID-19 (SARS-CoV-2) has a similar receptor-binding domain structure as SARS-CoV which suggests SARS-CoV-2 uses ACE2 receptor in humans for infection.
SARS-CoV-2 (COVID-19, 2019-nCoV) Structure
The structure[iii] of COVID-19 (SARS-CoV-2) consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E), a nucleocapsid protein (N), and RNA as seen in the figure below.
Spike protein (S) is heavily glycosylated, utilizes an N-terminal signal sequence to gain access to the ER and mediate attachment to host receptors. It is the largest structure and makes the distinct spikes on the surface of the virus. For SARS-CoV-2, S protein is cleaved by cellular furin-like protease TMPRSS2[iv] into two separate polypeptides S1 and S2. S1 also consists of a receptor binding domain(RBD) which binds to virus receptor ACE2
RNA is the genome of the virus.
Nucleocapsid protein (N) binds to RNA in vitro and is heavily phosphorylated. N proteins binds the viral genome in a beads on a string type conformation. This protein likely helps tether the viral genome to replicase-transcriptase complex (RTC), and subsequently package the encapsulated genome into viral particles.
Envelope protein (E) is found in small quantities in within the virus. It is most likely a transmembrane protein and with ion channel activity. The protein facilitates assembly and release of the virus and has other functions such as ion channel activity. It is not necessary for viral replication but it is for pathogenesis.
Membrane protein (M) is the most abundant structural protein. It does not contain signal sequence and exists as a dimer in the virion. It may have two different conformations to enable it to promote membrane curvature as well as bind to nucleocapsid.
Hemagglutinin-esterase dimer protein (HE) is present in a subset of betacoronaviruses. The protein binds sialic acids on surface glycoproteins. The protein activities are thought to enhance S protein-mediated cell entry and virus spread through the mucosa.
[i] World Health Organization "WHO Director-General's opening remarks at the media briefing on COVID-19 - 11 March 2020" https://www.who.int/dg/speeches/detail/who-director-general-s-opening-remarks-at-the-media-briefing-on-covid-19---11-march-2020
[ii] Lu R, Zhao X, Li J, et al. Genomic characterization and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. The Lancet. Published online January 29, 2020. https://doi.org/10.1016/ S0140-6736(20)30251-8
[iii] Fehr AR, Perlman S. Coronaviruses: an overview of their replication and pathogenesis. Methods Mol Biol. 2015;1282:1–23. https://doi.org/10.1007/978-1-4939-2438-7_1
[iv] Hoffmann, M., Kleine-Weber, H., Schroeder, S., et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. S0092–8674(20)30229–4 Advance online publication. https://doi.org/10.1016/j.cell.2020.02.052