HIGH PERFORMANCE ANTIBODIES … AND MORE

COVID-19 (SARS-CoV-2, 2019-nCoV) Antibodies

COVID-19 (SARS-CoV-2) ReagentsCOVID-19 (SARS-CoV-2) Background

COVID-19 (SARS-CoV-2) Virus Research Antibody Initiative

April 01, 2020, ProSci Inc is operational during this pandemic to support our customer. In response to the Coronavirus COVID-19 pandemic, ProSci Inc has developed and manufactured COVID-19 (SARS-CoV-2, 2019-nCoV) antibodies and research reagents against the virus, and virus receptor ACE2. Polyclonal antibodies and monoclonal antibodies are available for order against COVID-19 virus SARS-CoV-2 spike, envelope, and nucleocapsid protein. More polyclonal, monoclonal, and recombinant single domain antibodies are currently in our antibody production pipeline.

SARS-CoV-2 (COVID-19) Spike Protein ELISA       SARS-CoV-2 (COVID-19) Spike Protein Western Blot      

                                          

COVID-19 (SARS-CoV-2) Virus Reactive Antibodies

Antibody ProductsCat. No.HostApplicationClonalityProtein StructureBlocking Peptide
SARS-CoV-2/SARS-CoV Spike Antibody 3223 Rabbit ELISA, WB Polyclonal Spike Protein Blocking Peptide
SARS-CoV-2 (COVID-19)Spike Antibody 3525 Rabbit ELISA Polyclonal  Spike Protein Blocking Peptide
SARS-CoV-2 (COVID-19) Envelope Antibody 3531 Rabbit ELISA Polyclonal  Envelope Protein Blocking Peptide
SARS-CoV-2 (COVID-19) Nucleoprotein [3851] Antibody 35-579 Mouse ELISA, IF, WB Monoclonal  Nucleocapsid Protein N/A
SARS-CoV-2 (COVID-19) Nucleoprotein [3861] Antibody 35-580 Mouse ELISA, IF, WB Monoclonal  Nucleocapsid Protein N/A
SARS-CoV-2 (COVID-19) Nucleoprotein [3862] Antibody 35-710 Mouse ELISA, IF Monoclonal  Nucleocapsid Protein N/A
SARS-CoV-2 (COVID-19) Nucleoprotein [3863] Antibody 35-711 Mouse ELISA, IF Monoclonal  Nucleocapsid Protein N/A
SARS-CoV-2 (COVID-19) Nucleoprotein [3864] Antibody 35-712 Mouse ELISA, IF Monoclonal  Nucleocapsid Protein N/A

COVID-19 (SARS-CoV-2) Related Products

In addition to our COVID-19 (SARS-CoV-2) antibodies, ProSci scientists evaluated our SARS-CoV antibodies and determined the percentage of identity and homology between the immunogen used to develop these antibodies and the COVID-19 (SARS-CoV-2) sequences available as of February 15, 2020. Higher percentage of identity and homology suggests reactivity to  COVID-19 (SARS-CoV-2). Trial size antibodies are available for testing and a large batch is available to ensure lot to lot consistancy.  

COVID-19 (SARS-CoV-2) Recombinant Protein

ProductCat. No.SourceFusion Tag
2019-nCoV Nucleocapsid Recombinant Protein 97-077 E. Coli His Tag
2019-nCoV 3CL-Mpro Recombinant Protein 97-078 E. Coli His Tag
2019-nCoV S2 Recombinant Protein 97-079 HEK293 His Tag
2019-nCoV S RBD Recombinant Protein 97-080 HEK293 Fc Tag
2019-nCoV Envelope Recombinant Protein 97-082 E. Coli His Tag
SARS S1 Recombinant Protein 97-083 HEK293 His Tag
SARS S RBD Recombinant Protein 97-084 HEK293 His Tag

Coronavirus SARS-CoV Antibodies

ProductCat. No.HostApplicationClonalityProtein StructureIdentityHomologyBlocking Peptide
SARS Spike Antibody 3221 Rabbit ELISA Polyclonal Spike Protein 56% 69% Blocking Peptide
SARS Spike Antibody 3225 Rabbit ELISA Polyclonal Spike Protein 38% 56% Blocking Peptide
SARS Spike Antibody 3219 Rabbit ELISA Polyclonal Spike Protein 14% 29% Blocking Peptide
SARS Matrix Antibody 3527 Rabbit ELISA Polyclonal Membrane Protein 86% 93% Blocking Peptide
SARS Matrix Antibody 3529 Rabbit ELISA Polyclonal Membrane Protein 80% 93% Blocking Peptide
SARS Envelope Antibody 3533 Rabbit ELISA Polyclonal Envelope Protein 82% 91% Blocking Peptide


SARS-CoV-2 Receptor ACE2

    Custom Antibody Services

For COVID-19(SARS-CoV-2) receptor reagents, check out our ACE2 products! In addition to all our COVID-19 related antibody reagents, we also offer custom antibody services for polyclonal, monoclonal, and single domain antibodies.

COVID-19 (SARS-CoV-2) Background

What is COVID-19 ?

COVID-19 is an acute respiratory disease caused by novel coronavirus SARS-CoV-2  also known as 2019-nCoV. On March 11, 2020, the World Health Organization (WHO) characterized COVID-19 as a pandemic[i]

COVID-19 coronavirus SARS-CoV-2 belongs to the Betacoronavirus genus originating from bats. Betacoronaviruses can infect mammals, are zoonotic pathogens, and can cause severe respiratory disease in humans. Other viruses in this family are SARS coronavirus and MERS coronavirus. COVID-19 (SARS-CoV-2) has approximately 79% sequence identity to SARS-CoV and 50% to MERS-CoV.[ii] In addition, homology modeling shows COVID-19 (SARS-CoV-2)  has a similar receptor-binding domain structure as SARS-CoV which suggests COVID-19 (SARS-CoV-2) uses ACE2 receptor in humans for infection.  

COVID-19 (SARS-CoV-2) Structure

The structure[iii] of COVID-19 (SARS-CoV-2) consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE),  a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA as seen in the figure below.

ProSci COVID-19 (SARS-CoV-2) structure figure

Spike protein (S) is heavily glycosylated, utilizes an N-terminal signal sequence to gain access to the ER and mediate attachment to host receptors. It is the largest structure and makes the distinct spikes on the surface of the virus. For most coronaviruses, S protein is cleaved by a host cell furin-like protease into two separate polypeptides S1 and S2.

RNA is the genome of the virus.

Nucleocapsid protein (N) binds to RNA in vitro and is heavily phosphorylated. N proteins binds the viral genome in a beads on a string type conformation. This protein likely helps tether the viral genome to replicase-transcriptase complex (RTC), and subsequently package the encapsulated genome into viral particles.

Envelope protein (E) is found in small quantities in within the virus. It is most likely a transmembrane protein and with ion channel activity. The protein facilitates assembly and release of the virus and has other functions such as ion channel activity. It is not necessary for viral replication but it is for pathogenesis. 

Membrane protein (M) is the most abundant structural protein. It does not contain signal sequence and exists as a dimer in the virion. It may have two different conformations to enable it to promote membrane curvature as well as bind to nucleocapsid.

Hemagglutinin-esterase dimer protein (HE) is present in a subset of betacoronaviruses. The protein binds sialic acids on surface glycoproteins. The protein activities are thought to enhance S protein-mediated cell entry and virus spread through the mucosa.

References

[i] World Health Organization "WHO Director-General's opening remarks at the media briefing on COVID-19 - 11 March 2020" https://www.who.int/dg/speeches/detail/who-director-general-s-opening-remarks-at-the-media-briefing-on-covid-19---11-march-2020

[ii] Lu R, Zhao X, Li J, et al. Genomic characterization and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. The Lancet. Published online January 29, 2020. https://doi.org/10.1016/ S0140-6736(20)30251-8

[iii] Fehr AR, Perlman S. Coronaviruses: an overview of their replication and pathogenesis. Methods Mol Biol. 2015;1282:1–23. https://doi.org/10.1007/978-1-4939-2438-7_1