SARS-CoV-2 (COVID-19, 2019-nCoV) Research

SARS-CoV-2 (COVID-19, 2019-nCoV) research reagents - antibodies and proteins from ProSci Inc. - the SARS-CoV-2 structure. 

SARS-CoV-2 (COVID-19) Virus Antibody Research

ProSci Incorporated has developed and manufactured SARS-CoV-2 (COVID-19, 2019-nCoV) antibodies, antigens, and other reagents related to cellular entry: ACE2 receptor, TMPRSS2, and furin enzyme. ProSci Inc. is continuously adding new SARS-CoV-2 reagents for spike, S1, S2, nucleocapsid, membrane, envelope including llama single domain antibodies and recombinant proteins.

SARS-CoV-2 Featured Products

  COVID-19 Patient Validated 

Cat. No: 9099 SARS-CoV-2 (COVID-19) Nucleocapsid Antibody

Cat. No: 9083 SARS-CoV-2 (COVID-19) Spike S1 Antibody

SARS-CoV-2 (COVID-19) 9099 Nucleocapsid Protein IHC on COVID-19 Patient Lung | ProSci Inc.

  COVID-19 Patient Validated  

Cat. No: 3525 SARS-CoV-2 (COVID-19) Spike Antibody

COVID-19 (SARS-CoV-2) Patient Spike Protein IHC Validated | ProSci Inc.

  COVID-19 Patient Validated  

Cat. No: 3531 SARS-CoV-2 (COVID-19) Envelope Antibody

COVID-19 (SARS-CoV-2) 3531 Spike Protein ELISA | ProSci Inc.

SARS-CoV-2 (COVID-19) Antibody Pairs

ProSci Incorporated has launched matched SARS-CoV-2 (COVID-19, 2019-nCoV) antibody pairs validated by sandwich ELISA. These spike antibody pair and nucleocapsid antibody pairs are suitable for COVID-19 antigen test, ELISA, lateral flow and other immunoassay.

SARS-CoV-2 Nucleocapsid Matched Pair Antibodies

PairCapture Ab.HostClonalityDetection Ab.HostClonalityTarget ProteinBinding Protein
1 35-720 Mouse Monoclonal 9103 Rabbit Polyclonal Nucleocapsid 10-306
2 10-352 Rabbit Monoclonal 35-719 Mouse Monoclonal Nucleocapsid 10-306
3 10-352 Rabbit Monoclonal 35-720 Mouse Monoclonal Nucleocapsid 10-306

35-720/10-353 SARS-CoV-2 Nucleocapsid Protein 10-306 | ProSci Inc.

10-721/35-722 SARS-CoV-2 Nucleocapsid Protein 10-306 | ProSci Inc.

10-352/35-719 SARS-CoV-2 Nucleocapsid Protein 10-306 | ProSci Inc.

SARS-CoV-2 Spike Matched Pair Antibodies

PairCapture Ab.HostClonalityDetection Ab.HostClonalityTarget ProteinBinding Protein
1 10-351 Rabbit Monoclonal 10-706 Human Monoclonal Spike 97-087
2 10-351 Rabbit Monoclonal 9083-biotin Rabbit Polyclonal Spike 97-087
3 10-351 Rabbit Monoclonal 9091-biotin Rabbit Polyclonal Spike 97-087

10-351/9083-biotin SARS-CoV-2 Spike S1 Protein 97-087 | ProSci Inc.

10-350/9083-biotin SARS-CoV-2 Spike S1 Protein 97-087 | ProSci Inc.

10-351/9091-biotin SARS-CoV-2 Spike S1 Protein 97-087 | ProSci Inc.

New Product Pipeline for SARS-CoV-2

ProSci Incorporated continues to develop more SARS-CoV-2 (COVID-19, 2019-nCoV) antibodies and proteins against the full proteome of SARS-CoV-2, including Envelop, Matrix, NSPs and ORFs, which will be launched soon.

Ab TargetCat. No.
Envelope 9169
Membrane 9157, 9167
3CL-P/NSP5 9151, 9153
NSP7 9155
NSP8 9159, 9167
NSP9 9161, 9163
NSP2 9171, 9173
NSP4 9175
NSP6 9177
NSP10 9179
NSP13 9181, 9183
NSP14 9185, 9187
Ab TargetCat. No.
ORF6 9189
ORF9b 9191
NSP12 9267
NSP15 9269
NSP16 9271
ORF3a Soon to be released
ORF3b Soon to be released
ORF7a Soon to be released
ORF8 Soon to be released
ORF9c Soon to be released
ORF10 Soon to be released

SARS-CoV-2 (COVID-19, 2019-nCoV) Antibody Reagents

ProSci offers a comprehensive list of SARS-CoV-2 antibodies against viral proteins; spike, nucleocapsid, envelope, ORF, and NSP. These antibodies can be used with various applications: IHC, Flow Cytometry, ELISA, WB, and Lateral-Flow.

Diagram of SARS-CoV-2 with Validated Antibodies

SARS-COV-2 with Validated Antibodies Diagram | ProSci Inc.

TargetSARS-CoV-2 Structure Protein Antibodies
Spike S1 9083 / 9091 / 10-350 / 10-351 / 10-703 / 10-706 / ...
Spike RBD 9087 / 93-309 / 10-713 / 10-715 / 10-052 / 10-059 / ...
Spike S2 3525 / 9123 / 9119
Spike ECD 24-032 / 10-528 / 10-534
Nucleocapsid 9099 / 9103 / 35-579 / 35-719 / 10-352 / 10-353 / ...
Envelope 3531 / 9169 / 10-518
Membrane 9165 / 9157 / 10-516

Diagram of ProSci Inc. Antibodies against SARS-CoV-2 Proteome

ProSci Antibodies against SARS-CoV-2 Proteome Diagram | ProSci Inc.

TargetSARS-CoV-2 ORF/NSP Antibodies
ORF3a 10-519 / 10-520 / 10-521 /
ORF6 9189 / 10-515
ORF7a 10-513 / 10-514
ORF8 10-511 / 10-512 / 10-532
ORF9b 9191
ORF10 10-507
NSP1 10-500
NSP2 9171, 9173
PPL-P/NSP3 10-504 / 10-505 / 10-506
NSP4 9175
3CL-P/NSP5 9151, 9153
TargetSARS-CoV-2 NSP Antibodies
NSP6 9177
NSP7 9155
NSP8 9159, 9167
NSP9 9161, 9163
NSP10 9179
NSP12 Soon to be released
NSP13 9181, 9183
NSP14 9185, 9187
NSP15 Soon to be released
NSP16 Soon to be released

SARS-CoV-2 (COVID-19, 2019-nCoV) Recombinant Proteins

Recombinant proteins developed in-house & tested with validated antibodies. Our recombinant proteins expressed are in various cell lines and expression systems for the novel coronavirus SARS-CoV-2 (COVID-19, 2019-nCoV): Full/partial length, tagged/untagged, and biologically active.

Diagram of SARS-CoV-2 Proteins

SARS-COV-2 Proteins Diagram | ProSci Inc.

TargetSARS-CoV-2 Structure Protein
Spike S1 10-422 / 10-423 / 97-087 / ...
Spike RBD 10-303 / 10-433 / 97-093 / ...
Spike S2 10-426 / 10-115
Spike ECD 10-108 / 10-121 / 10-122
Nucleocapsid 10-306 / 10-434 / 97-082 / ...
Envelope 10-112 / 97-082
Membrane 10-429

Diagram of ProSci Inc. Proteins against SARS-CoV-2 Proteome

ProSci Proteins against SARS-CoV-2 Proteome Diagram | ProSci Inc

TargetSARS-CoV-2 ORF/NSP Protein
ORF3a 10-005
ORF6 10-437
ORF7a 10-435
ORF8 10-002 / 10-436
NSP1 10-416 / 97-095
NSP2 10-425
PPL-P/NSP3 10-404 / 10-406 / 10-119
3CL-P/NSP5 10-400 / 10-412 / 10-116
TargetSARS-CoV-2 NSP Protein
NSP7 97-096
NSP8 10-415 / 97-097
NSP9 10-417
NSP10 10-408
NSP12 10-419
NSP13 10-427
NSP15 10-420
NSP16 10-418

SARS-CoV Reagents

In addition to our coronavirus COVID-19 (SARS-CoV-2) antibodies & recombinant proteins, similar related reagents are available for SARS-CoV.  The two viruses, SARS-CoV-2 and SARS-CoV, have approximately 79% sequence identity. For potential antibody cross reactivity refer to the table below.   

ProSci Inc. SARS-CoV Antibodies Diagram

SARS-CoV ProSci Antibodies Diagram | ProSci Inc.

SARS-CoV Antibodies

ProductCat. No.HostApplicationClonalityProtein StructureIdentityHomologyBlocking Peptide
SARS Spike Antibody 3221 Rabbit ELISA Polyclonal Spike Protein 56% 69% Blocking Peptide
SARS Spike Antibody 3225 Rabbit ELISA Polyclonal Spike Protein 38% 56% Blocking Peptide
SARS Spike Antibody 3219 Rabbit ELISA Polyclonal Spike Protein 14% 29% Blocking Peptide
SARS Matrix Antibody 3527 Rabbit ELISA Polyclonal Membrane Protein 86% 93% Blocking Peptide
SARS Matrix Antibody 3529 Rabbit ELISA Polyclonal Membrane Protein 80% 93% Blocking Peptide
SARS Envelope Antibody 3533 Rabbit ELISA Polyclonal Envelope Protein 82% 91% Blocking Peptide

SARS-CoV Recombinant Protein

ProductCat. No.SourceFusion TagSequence
SARS S Recombinant Protein 97-094 HEK293 His Tag Ser 14 - Pro 1195

SARS-CoV-2 Receptor ACE2 | ProSci Inc

    Custom Antibody Services | ProSci Inc

For COVID-19 (SARS-CoV-2) receptor reagents, check out our ACE2 products! In addition to all our COVID-19 related antibody reagents, we also offer custom antibody services for polyclonal, monoclonal, and single domain antibodies.

What is SARS-CoV-2?

SARS-CoV-2 (COVID-19, 2019-nCoV) is a major public heatlh threat with more than 1,200,000 deaths worldwide. The first stage in the coronavirus life cycle is viral entry where the viral spike protein will bind to a specific cell surface receptor. The spike proteins of SARS-CoV-2, as well as SARS-CoV-1 and NL63 use angiotensin-converting enzyme 2 (ACE2) as a receptor for binding. An antibody for this ACE2 protein has been produced by ProSci Inc. (Catalog No. 3217) that has been shown to function in immunohistochemistry and immunofluorescence experiments in human samples, and western blot experiments in human, mouse, and rat samples.

SARS-CoV-2 (COVID-19, 2019-nCoV) Background

COVID-19 is an acute respiratory disease caused by novel coronavirus SARS-CoV-2  also known as 2019-nCoV. On March 11, 2020, the World Health Organization (WHO) characterized COVID-19 as a pandemic[i]COVID-19 coronavirus SARS-CoV-2 belongs to the Betacoronavirus genus originating from bats. Betacoronaviruses can infect mammals, are zoonotic pathogens, and can cause severe respiratory disease in humans. Other viruses in this family are SARS coronavirus and MERS coronavirus. COVID-19 (SARS-CoV-2) has approximately 79% sequence identity to SARS-CoV and 50% to MERS-CoV.[ii] In addition, homology modeling shows COVID-19 (SARS-CoV-2)  has a similar receptor-binding domain structure as SARS-CoV which suggests SARS-CoV-2 uses ACE2 receptor in humans for infection.  

SARS-CoV-2 (COVID-19, 2019-nCoV) Structure

The structure[iii] of COVID-19 (SARS-CoV-2) consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E), a nucleocapsid protein (N), and RNA as seen in the figure below.

ProSci Inc. COVID-19 (SARS-CoV-2) structure figure | ProSci Inc.

Spike protein (S) is heavily glycosylated, utilizes an N-terminal signal sequence to gain access to the ER and mediate attachment to host receptors. It is the largest structure and makes the distinct spikes on the surface of the virus. For SARS-CoV-2, S protein is cleaved by cellular furin-like protease TMPRSS2[iv] into two separate polypeptides S1 and S2. S1 also consists of a receptor binding domain(RBD) which binds to virus receptor ACE2

RNA is the genome of the virus.

Nucleocapsid protein (N) binds to RNA in vitro and is heavily phosphorylated. N proteins binds the viral genome in a beads on a string type conformation. This protein likely helps tether the viral genome to replicase-transcriptase complex (RTC), and subsequently package the encapsulated genome into viral particles.

Envelope protein (E) is found in small quantities in within the virus. It is most likely a transmembrane protein and with ion channel activity. The protein facilitates assembly and release of the virus and has other functions such as ion channel activity. It is not necessary for viral replication but it is for pathogenesis. 

Membrane protein (M) is the most abundant structural protein. It does not contain signal sequence and exists as a dimer in the virion. It may have two different conformations to enable it to promote membrane curvature as well as bind to nucleocapsid.

Hemagglutinin-esterase dimer protein (HE) is present in a subset of betacoronaviruses. The protein binds sialic acids on surface glycoproteins. The protein activities are thought to enhance S protein-mediated cell entry and virus spread through the mucosa.


[i] World Health Organization "WHO Director-General's opening remarks at the media briefing on COVID-19 - 11 March 2020"

[ii] Lu R, Zhao X, Li J, et al. Genomic characterization and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. The Lancet. Published online January 29, 2020. S0140-6736(20)30251-8

[iii] Fehr AR, Perlman S. Coronaviruses: an overview of their replication and pathogenesis. Methods Mol Biol. 2015;1282:1–23.

[iv] Hoffmann, M., Kleine-Weber, H., Schroeder, S., et al.   SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. S0092–8674(20)30229–4 Advance online publication.