Connexin 43 (phospho Ser368) Antibody Cat. No.: 50-220

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Bovine, Chicken, Dog, Goat, Human, Mouse, Rat, Zebrafish
IMMUNOGEN: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser368 of rat connexin 43.
TESTED APPLICATIONS: WB
APPLICATIONS: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, guinea pig, human, mouse and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
PREDICTED MOLECULAR WEIGHT: 43

psi-iconProperties
PURIFICATION:Affinity Purified
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
STORAGE CONDITIONS:Connexin 43 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

psi-iconAdditional Info
OFFICIAL SYMBOL:Gja1
ALTERNATE NAMES:Cx43, Cxn-43, Connexin-43, Cx43
ACCESSION NO.:P08050
PROTEIN GI NO.:117708
GENE ID:24392
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin 43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin 43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin 43 that results in a decrease in gap junction permeability (Bao et al., 2004b).
REFERENCES:1) Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459.
2) Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654.
3) Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066.

ANTIBODIES FOR RESEARCH USE ONLY.

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