Complement C5 Recombinant Protein Cat. No.: 11-353

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psi-iconSpecifications
SPECIES:Rat
SOURCE SPECIES:HEK293 cells
SEQUENCE:Gln 24 - Ala 1688
FUSION TAG:His Tag
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 74.1 kDa (β chain) and 114.8 kDa (α
psi-iconProperties
PURITY:>95% as determined by SDS-PAGE.
PREDICTED MOLECULAR WEIGHT:74.1 kDa (β chain) & 114.8 kDa (α chain)
PHYSICAL STATE:Lyophilized
BUFFER:PBS, pH7.4
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:Complement C5,C5,CPAMD4
ACCESSION NO.:XP_001079130.2
psi-iconBackground and References
BACKGROUND:Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
REFERENCES:1) DeMartino JA, et al. 1994. J Biol Chem. 269(20):14446-50.
2) Fredslund F, et al. 2008. Nat Immunol. 9(7):753-60.
3) Chen Z, et al. 1998. J Biol Chem. 273(17):10411-9.

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