|SOURCE:||Rabbit colon tissue|
|APPLICATIONS:||Colon lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.|
|BUFFER:||HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.|
|CONCENTRATION:||2 mg/mL or better|
|STORAGE CONDITIONS:||Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.|
|Rabbit colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The rabbit colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.|
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For Research Use Only